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Valosin-containing protein (VCP/p97) plays a role in the replication of West Nile virus
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| Title: | Valosin-containing protein (VCP/p97) plays a role in the replication of West Nile virus |
| Authors: | Phongphaew, Wallaya Browse this author | | Kobayashi, Shintaro Browse this author →KAKEN DB | | Sasaki, Michihito Browse this author →KAKEN DB | | Carr, Michael Browse this author →KAKEN DB | | Hall, William W. Browse this author | | Orba, Yasuko Browse this author →KAKEN DB | | Sawa, Hirofumi Browse this author →KAKEN DB |
| Keywords: | VCP | | WNV replication | | Early steps | | Genome replication |
| Issue Date: | 15-Jan-2017 |
| Publisher: | Elsevier |
| Journal Title: | Virus Research |
| Volume: | 228 |
| Start Page: | 114 |
| End Page: | 123 |
| Publisher DOI: | 10.1016/j.virusres.2016.11.029 |
| PMID: | 27914931 |
| Abstract: | Valosin-containing protein (VCP) is classified as a member of the type II AAA(+) ATPase protein family. VCP functions in several cellular processes, including protein degradation, membrane fusion, vesicular trafficking and disassembly of stress granules. Moreover, VCP is considered to play a role in the replication of several viruses, albeit through different mechanisms. In the present study, we have investigated the role of VCP in West Nile virus (WNV) infection. Endogenous VCP expression was inhibited using either VCP inhibitors or by siRNA knockdown. It could be shown that the inhibition of endogenous VCP expression significantly inhibited WNV infection. The entry assay revealed that silencing of endogenous VCP caused a significant reduction in the expression levels of WNV-RNA compared to control siRNA-treated cells. This indicates that VCP may play a role in early steps either the binding or entry steps of the WNV life cycle. Using WNV virus like particles and WNV-DNA-based replicon, it could be demonstrated that perturbation of VCP expression decreased levels of newly synthesized WNV genomic RNA. These findings suggest that VCP is involved in early steps and during genome replication of the WNV life cycle. |
| Rights: | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/67761 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 澤 洋文
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