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Valosin-containing protein (VCP/p97) plays a role in the replication of West Nile virus

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Title: Valosin-containing protein (VCP/p97) plays a role in the replication of West Nile virus
Authors: Phongphaew, Wallaya Browse this author
Kobayashi, Shintaro Browse this author →KAKEN DB
Sasaki, Michihito Browse this author →KAKEN DB
Carr, Michael Browse this author →KAKEN DB
Hall, William W. Browse this author
Orba, Yasuko Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Keywords: VCP
WNV replication
Early steps
Genome replication
Issue Date: 15-Jan-2017
Publisher: Elsevier
Journal Title: Virus Research
Volume: 228
Start Page: 114
End Page: 123
Publisher DOI: 10.1016/j.virusres.2016.11.029
PMID: 27914931
Abstract: Valosin-containing protein (VCP) is classified as a member of the type II AAA(+) ATPase protein family. VCP functions in several cellular processes, including protein degradation, membrane fusion, vesicular trafficking and disassembly of stress granules. Moreover, VCP is considered to play a role in the replication of several viruses, albeit through different mechanisms. In the present study, we have investigated the role of VCP in West Nile virus (WNV) infection. Endogenous VCP expression was inhibited using either VCP inhibitors or by siRNA knockdown. It could be shown that the inhibition of endogenous VCP expression significantly inhibited WNV infection. The entry assay revealed that silencing of endogenous VCP caused a significant reduction in the expression levels of WNV-RNA compared to control siRNA-treated cells. This indicates that VCP may play a role in early steps either the binding or entry steps of the WNV life cycle. Using WNV virus like particles and WNV-DNA-based replicon, it could be demonstrated that perturbation of VCP expression decreased levels of newly synthesized WNV genomic RNA. These findings suggest that VCP is involved in early steps and during genome replication of the WNV life cycle.
Rights: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 澤 洋文

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