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Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
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| Title: | Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor |
| Authors: | Mikuni, Shintaro Browse this author | | Yamamoto, Johtaro Browse this author | | Horio, Takashi Browse this author | | Kinjo, Masataka Browse this author →KAKEN DB |
| Keywords: | raster image correlation spectroscopy | | green fluorescence protein | | nuclear receptor | | diffusion analysis | | transcription |
| Issue Date: | Sep-2017 |
| Publisher: | MDPI |
| Journal Title: | International Journal of Molecular Sciences |
| Volume: | 18 |
| Issue: | 9 |
| Start Page: | 1855 |
| Publisher DOI: | 10.3390/ijms18091855 |
| Abstract: | The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively characterize the diffusion properties of EGFP labeled human GR (EGFP-hGR) and its mutants in the cell nucleus. RICS is an image correlation technique that evaluates the spatial distribution of the diffusion coefficient as a diffusion map. Interestingly, we observed that the averaged diffusion coefficient of EGFP-hGR strongly and negatively correlated with its transcriptional activities in comparison to that of EGFP-hGR wild type and mutants with various transcriptional activities. This result suggests that the decreasing of the diffusion coefficient of hGR was reflected in the high-affinity binding to DNA. Moreover, the hyper-phosphorylation of hGR can enhance the transcriptional activity by reduction of the interaction between the hGR and the nuclear corepressors. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/67846 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 金城 政孝
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