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Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/67846

Title: Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor
Authors: Mikuni, Shintaro Browse this author
Yamamoto, Johtaro Browse this author
Horio, Takashi Browse this author
Kinjo, Masataka Browse this author →KAKEN DB
Keywords: raster image correlation spectroscopy
green fluorescence protein
nuclear receptor
diffusion analysis
transcription
Issue Date: Sep-2017
Publisher: MDPI
Journal Title: International Journal of Molecular Sciences
Volume: 18
Issue: 9
Start Page: 1855
Publisher DOI: 10.3390/ijms18091855
Abstract: The glucocorticoid receptor (GR) is a transcription factor, which interacts with DNA and other cofactors to regulate gene transcription. Binding to other partners in the cell nucleus alters the diffusion properties of GR. Raster image correlation spectroscopy (RICS) was applied to quantitatively characterize the diffusion properties of EGFP labeled human GR (EGFP-hGR) and its mutants in the cell nucleus. RICS is an image correlation technique that evaluates the spatial distribution of the diffusion coefficient as a diffusion map. Interestingly, we observed that the averaged diffusion coefficient of EGFP-hGR strongly and negatively correlated with its transcriptional activities in comparison to that of EGFP-hGR wild type and mutants with various transcriptional activities. This result suggests that the decreasing of the diffusion coefficient of hGR was reflected in the high-affinity binding to DNA. Moreover, the hyper-phosphorylation of hGR can enhance the transcriptional activity by reduction of the interaction between the hGR and the nuclear corepressors.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/67846
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 金城 政孝

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