| Title: | Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis |
| Authors: | Goto, Shinya Browse this author |
| Konnai, Satoru Browse this author →KAKEN DB |
| Okagawa, Tomohiro Browse this author |
| Nishimori, Asami Browse this author |
| Maekawa, Naoya Browse this author |
| Gondaira, Satoshi Browse this author |
| Higuchi, Hidetoshi Browse this author |
| Koiwa, Masateru Browse this author →KAKEN DB |
| Tajima, Motoshi Browse this author →KAKEN DB |
| Kohara, Junko Browse this author |
| Ogasawara, Satoshi Browse this author →KAKEN DB |
| Kato, Yukinari Browse this author |
| Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Murata, Shiro Browse this author →KAKEN DB |
| Ohashi, Kazuhiko Browse this author →KAKEN DB |
| Keywords: | Bovine mycoplasmosis |
| IFN-γ |
| immunosuppression |
| PD-1 |
| PD-L1 |
| Issue Date: | Sep-2017 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Immunity, inflammation and disease |
| Volume: | 5 |
| Issue: | 3 |
| Start Page: | 355 |
| End Page: | 363 |
| Publisher DOI: | 10.1002/iid3.173 |
| Abstract: | Introduction:Bovine mycoplasma, chieflyMycoplasma bovis, is a pathogen thatcauses pneumonia, mastitis, arthritis, and otitis media in cattle. This pathogenexerts immunosuppressive effects, such as the inhibition of interferon production.However, the mechanisms involved in bovine mycoplasmosis have not been fullyelucidated. In this study, we investigated the role of the programmed death-1(PD-1)/programmed death-ligand 1 (PD-L1) pathway in immunosuppression inbovine mycoplasmosis.Methods:In the initial experiments, we used enzyme-linked immunosorbent assayto measure interferon-g(IFN-g) from peripheral blood mononuclear cells(PBMCs) isolated from cattle with mycoplasmosis.Results:Expectedly, IFN-gproduction significantly decreased in cattle withmycoplasmosis compared with that in clinically healthy cattle. Concomitantly,flow cytometric analysis revealed that the proportions of PD-1tCD4tandPD-L1tCD14tcells significantly increased in peripheral blood of the infectedcattle. Interestingly, the number of PD-1tCD4tand PD-1tCD8tT cells werenegatively correlated with IFN-gproduction from PBMCs in bovine mycoplas-mosis. Additionally, blockade of the PD-1/PD-L1 pathway in vitro by anti-bovinePD-1- and anti-bovine PD-L1 antibodies significantly upregulated the productionof IFN-gfrom anti-mycoplasma-specific cells.Conclusions:These results suggest that the PD-1/PD-L1 pathway could beinvolved in immune exhaustion of bovine mycoplasma-specific T cells. Inconclusion, our study opens up a new perspective in the therapeutic strategy forbovine mycoplasmosis by targeting the immunoinhibitory receptor pathways |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/67897 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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