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Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis

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Title: Increase of cells expressing PD-1 and PD-L1 and enhancement of IFN-γ production via PD-1/PD-L1 blockade in bovine mycoplasmosis
Authors: Goto, Shinya Browse this author
Konnai, Satoru Browse this author →KAKEN DB
Okagawa, Tomohiro Browse this author
Nishimori, Asami Browse this author
Maekawa, Naoya Browse this author
Gondaira, Satoshi Browse this author
Higuchi, Hidetoshi Browse this author
Koiwa, Masateru Browse this author →KAKEN DB
Tajima, Motoshi Browse this author →KAKEN DB
Kohara, Junko Browse this author
Ogasawara, Satoshi Browse this author →KAKEN DB
Kato, Yukinari Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Murata, Shiro Browse this author →KAKEN DB
Ohashi, Kazuhiko Browse this author →KAKEN DB
Keywords: Bovine mycoplasmosis
IFN-γ
immunosuppression
PD-1
PD-L1
Issue Date: Sep-2017
Publisher: John Wiley & Sons
Journal Title: Immunity, inflammation and disease
Volume: 5
Issue: 3
Start Page: 355
End Page: 363
Publisher DOI: 10.1002/iid3.173
Abstract: Introduction:Bovine mycoplasma, chieflyMycoplasma bovis, is a pathogen thatcauses pneumonia, mastitis, arthritis, and otitis media in cattle. This pathogenexerts immunosuppressive effects, such as the inhibition of interferon production.However, the mechanisms involved in bovine mycoplasmosis have not been fullyelucidated. In this study, we investigated the role of the programmed death-1(PD-1)/programmed death-ligand 1 (PD-L1) pathway in immunosuppression inbovine mycoplasmosis.Methods:In the initial experiments, we used enzyme-linked immunosorbent assayto measure interferon-g(IFN-g) from peripheral blood mononuclear cells(PBMCs) isolated from cattle with mycoplasmosis.Results:Expectedly, IFN-gproduction significantly decreased in cattle withmycoplasmosis compared with that in clinically healthy cattle. Concomitantly,flow cytometric analysis revealed that the proportions of PD-1tCD4tandPD-L1tCD14tcells significantly increased in peripheral blood of the infectedcattle. Interestingly, the number of PD-1tCD4tand PD-1tCD8tT cells werenegatively correlated with IFN-gproduction from PBMCs in bovine mycoplas-mosis. Additionally, blockade of the PD-1/PD-L1 pathway in vitro by anti-bovinePD-1- and anti-bovine PD-L1 antibodies significantly upregulated the productionof IFN-gfrom anti-mycoplasma-specific cells.Conclusions:These results suggest that the PD-1/PD-L1 pathway could beinvolved in immune exhaustion of bovine mycoplasma-specific T cells. Inconclusion, our study opens up a new perspective in the therapeutic strategy forbovine mycoplasmosis by targeting the immunoinhibitory receptor pathways
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/67897
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 定彦

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