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Pyridoxine supplementation during oocyte maturation improves the development and quality of bovine preimplantation embryos

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Title: Pyridoxine supplementation during oocyte maturation improves the development and quality of bovine preimplantation embryos
Authors: Aboelenain, Mansour Browse this author
Balboula, Ahmed Zaky Browse this author
Kawahara, Manabu Browse this author →KAKEN DB
Montaser, Abd El-Monem Browse this author
Zaabel, Samy Moawad Browse this author
Kim, Sung-Woo Browse this author
Nagano, Masashi Browse this author →KAKEN DB
Takahashi, Masashi Browse this author →KAKEN DB
Keywords: Cathepsin B
Pyridoxine
In vitro maturation
Developmental competence
Bovine oocytes
Issue Date: 15-Mar-2017
Publisher: Elsevier
Journal Title: Theriogenology
Volume: 91
Start Page: 127
End Page: 133
Publisher DOI: 10.1016/j.theriogenology.2016.12.022
PMID: 28215677
Abstract: Recently, inhibition of cathepsin B (CTSB) activity during in vitro maturation (IVM) and culture (NC) improved the developmental competence and quality of bovine oocytes and embryos. E-64 is a widely used inhibitor to inhibit CTSB activity, however, E-64 inhibits not only CTSB activity but also the activities of other proteases including cathepsin L (CTSL), papain, calpain, and trypsin. Pyridoxine, the catalytically active form of vitamin B6, plays a crucial role in several cellular processes and has the ability to inhibit CTSB activity. However, whether pyridoxine has an improving effect during IVM of bovine oocytes is still unknown. In this study, we investigated the effect of pyridoxine supplementation during IVM on the developmental competence of bovine oocytes and the quality of the produced blastocysts. Supplementation of pyridoxine to the maturation medium significantly decreased the activity of CTSB in both bovine cumulus cells and oocytes. Moreover, pyridoxine improved both the blastocyst and hatched blastocyst rates. In addition, the presence of pyridoxine during IVM also significantly improved the quality of the produced embryos by increasing the total cell number as well as decreasing the CTSB mRNA expression and apoptotic rate. These results indicate that pyridoxine is a promising tool to improve the developmental competence of bovine oocytes and subsequent embryo quality.
Rights: © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/67967
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 昌志

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