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How much medium do you use for cell culture? Medium volume influences mineralization and osteoclastogenesis in vitro

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Title: How much medium do you use for cell culture? Medium volume influences mineralization and osteoclastogenesis in vitro
Authors: Yoshimura, Yoshitaka Browse this author →KAKEN DB
Kikuiri, Takashi Browse this author →KAKEN DB
Hasegawa, Tomokazu Browse this author →KAKEN DB
Matsuno, Mino Browse this author →KAKEN DB
Minamikawa, Hajime Browse this author
Deyama, Yoshiaki Browse this author →KAKEN DB
Suzuki, Kuniaki Browse this author →KAKEN DB
Keywords: medium volume
mineralization
osteoclastogenesis
osteoblast
osteoclast
Issue Date: Jul-2017
Publisher: Spandidos Publications
Journal Title: Molecular medicine reports
Volume: 16
Issue: 1
Start Page: 429
End Page: 434
Publisher DOI: 10.3892/mmr.2017.6611
PMID: 28535008
Abstract: Bone is maintained by a balance between bone formation and resorption. This remodeling is controlled by a wide variety of systemic and local factors including hormones, cytokines and mechanical stresses. The present in vitro study examined the impact of medium volume, using 0.4, 0.6, 0.8, 1.0, 1.5 and 2.0 ml/well in a 24-well plate, on the differentiation of osteoblasts and osteoclasts. There were no differences in the alkaline phosphatase activity of osteoblasts amongst the groups; however, the area of mineral deposition was decreased in a media volume-dependent manner. A co-culture of osteoblastic cells with bone marrow cells revealed a reduction in the total number of osteoclastic tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells (>= 2 nuclei), whereas the formation of large osteoclastic TRAP-positive multinuclear cells (>= 8 nuclei) was increased, in a media volume-dependent manner. There were also no differences in receptor activator of nuclear factor-kappa B ligand mRNA and total osteoprotegerin (OPG) protein expression levels amongst the groups, however the concentration of OPG decreased in a media volume-dependent manner. In conclusion, the present study demonstrated that the suppression of mineralization in osteoblastic cells and the stimulation of osteoclast fusion are dependent on the medium volume, indicating that media volume is an important factor in in vitro cell culture systems.
Type: article
URI: http://hdl.handle.net/2115/68022
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉村 善隆

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