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Association between nephrinuria, podocyturia, and proteinuria in women with pre-eclampsia

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Title: Association between nephrinuria, podocyturia, and proteinuria in women with pre-eclampsia
Authors: Furuta, Itsuko Browse this author →KAKEN DB
Zhai, Tianyue Browse this author
Ishikawa, Satoshi Browse this author
Umazume, Takeshi Browse this author →KAKEN DB
Nakagawa, Kinuko Browse this author
Yamada, Takahiro Browse this author →KAKEN DB
Morikawa, Mamoru Browse this author →KAKEN DB
Minakami, Hisanori Browse this author →KAKEN DB
Keywords: nephrin
podocyte injury
pre-eclampsia
proteinuric diseases
urine test
Issue Date: Jan-2017
Publisher: Wiley-Blackwell
Journal Title: Journal of Obstetrics and Gynaecology Research
Volume: 43
Issue: 1
Start Page: 34
End Page: 41
Publisher DOI: 10.1111/jog.13180
PMID: 27862669
Abstract: Aim: Podocyte depletion in the kidney is associated with end-stage kidney disease (ESKD). Pre-eclampsia (PE) increases the risk of ESKD in later life. This study was performed to determine whether nephrinuria (soluble nephrin in the urine) is correlated with proteinuria and/or podocyturia (podocytes in the urine) in PE women. Methods: Eighty-three urine samples, consisting of 45 and 38 samples from 27 normotensive and nine PE women, respectively, underwent simultaneous determination of nephrin, protein, and creatinine concentrations in the urine supernatant and quantitative analysis of podocyte-specific protein mRNA expression. This included podocin (Pod-mRNA) and nephrin (Nep-mRNA), using real-time polymerase chain reaction in the pelleted urine. Nephrinuria and proteinuria were corrected by creatinine concentration. Pod- and Nep-mRNA expression levels were corrected by GAPDH. Results: Nephrinuria, proteinuria, Pod-mRNA expression, and Nep-mRNA expression all increased with advancing gestation in PE women, while not in normotensive women. The nephrinuria was strongly correlated with proteinuria (R = 0.901, P < 0.001), Pod-mRNA expression level (R = 0.824, P < 0.001), and Nep-mRNA expression level (R = 0.724, P < 0.001) in urine samples from PE women, while the nephrinuria was significantly correlated with proteinuria alone (R = 0.419, P < 0.005) in urine samples from normotensive women. Conclusion: Nephrinuria reflected well the degrees of proteinuria and podocyturia in PE women. This suggested that increased nephrinuria/proteinuria was associated with podocyte loss in the kidneys of PE women.
Rights: This is the peer reviewed version of the following article: Furuta, I., Zhai, T., Ishikawa, S., Umazume, T., Nakagawa, K., Yamada, T., Morikawa, M., and Minakami, H. (2017) Association between nephrinuria, podocyturia, and proteinuria in women with pre-eclampsia. J. Obstet. Gynaecol. Res., 43: 34–41, which has been published in final form at http://dx.doi.org/10.1111/jog.13180. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/68034
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 古田 伊都子

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