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Synthetic Mucin-Like Glycopeptides as Versatile Tools to Measure Effects of Glycan Structure/Density/Position on the Interaction with Adhesion/Growth-Regulatory Galectins in Arrays

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この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/68039

タイトル: Synthetic Mucin-Like Glycopeptides as Versatile Tools to Measure Effects of Glycan Structure/Density/Position on the Interaction with Adhesion/Growth-Regulatory Galectins in Arrays
著者: Artigas, Gerard 著作を一覧する
Hinou, Hiroshi 著作を一覧する
Garcia-Martin, Fayna 著作を一覧する
Gabius, Hans-Joachim 著作を一覧する
Nishimura, Shin-Ichiro 著作を一覧する
キーワード: agglutinin
glycoproteins
glycosylation
lectin
TF antigen
発行日: 2017年 1月 3日
出版者: Wiley-Blackwell
誌名: Chemistry-an asian journal
巻: 12
号: 1
開始ページ: 159
終了ページ: 167
出版社 DOI: 10.1002/asia.201601420
PMID: 27873468
抄録: Functional pairing of cellular glycoconjugates with tissue lectins is a highly selective process, whose determinative factors have not yet been fully delineated. Glycan structure and modes of presentation, that is, its position and density, can contribute to binding, as different members of a lectin family can regulate degrees of responsiveness to these factors. Using a peptide repeat sequence motif of the glycoprotein mucin-1, the principle of introducing synthetic (glyco) peptides with distinct variations in these three parameters to an array-based screening of tissue lectins is illustrated. Interaction profiles of seven adhesion/growth-regulatory galectins cover the range from intense signals with core 2 pentasaccharides and core 1 binding for galectins-3 and -5 to a lack of binding for galectin-1 and also the galectin-related protein, which was included as a negative control. Remarkably, the two tandem-repeat-type galectins-4 and -8 were distinguished by core 1 sialylation, as the two separated domains were. These results encourage further synthetic elaboration of the glycopeptide library and testing of the network of natural galectins and rationally engineered variants of the lectins.
Rights: This is the peer reviewed version of the following article: G. Artigas, H. Hinou, F. Garcia-Martin, H.-J. Gabius, S.-I. Nishimura, Synthetic Mucin-Like Glycopeptides as Versatile Tools to Measure Effects of Glycan Structure/Density/Position on the Interaction with Adhesion/Growth-Regulatory Galectins in Arrays, Chem. Asian J. 2017, 12, 159-167, which has been published in final form at 10.1002/asia.201601420. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/68039
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 比能 洋

 

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