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Roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse ontogeny

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Title: Roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse ontogeny
Authors: Kawahara, Manabu Browse this author →KAKEN DB
Kono, Tomohiro Browse this author
Keywords: Development
Dlk1
Genomic imprinting
Gtl2
H19
Igf2
Mouse
Issue Date: Apr-2012
Publisher: 日本繁殖生物学会
Journal Title: Journal of Reproduction and Development
Volume: 58
Issue: 2
Start Page: 175
End Page: 179
Publisher DOI: 10.1262/jrd.2011-053
Abstract: We studied the longevity of mice produced without sperm using the genomes of oocytes that are already committed to a germline cell lineage. The first sperm-free mouse “KAGUYA”, which we term ‘bi-maternal mouse’, was born on 3 February, 2003. Bi-maternal embryos were generated using 2 sets of female genomes—one derived from fully grown oocytes from normal adults and the other from non-growing oocytes from newborn pups. These genomes were combined by nuclear transfer. We refined the technique for generating bi-maternal mice and found that genetic manipulations in only 2 regions―the imprinting centres of Igf2-H19 and Dlk1-Gtl2―on chromosomes 7 and 12 of the newborn pups allowed us to generate bi-maternal mice at a high rate. Studying bi-maternal conceptuses and mice provides further insight into the mechanisms by which paternally methylated imprinted genes regulate mammalian ontogenesis.
Description: SRD Young Investigator Award 2011
Type: article
URI: http://hdl.handle.net/2115/68132
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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