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Roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse ontogeny
Title: | Roles of genes regulated by two paternally methylated imprinted regions on chromosomes 7 and 12 in mouse ontogeny |
Authors: | Kawahara, Manabu Browse this author →KAKEN DB | Kono, Tomohiro Browse this author |
Keywords: | Development | Dlk1 | Genomic imprinting | Gtl2 | H19 | Igf2 | Mouse |
Issue Date: | Apr-2012 |
Publisher: | 日本繁殖生物学会 |
Journal Title: | Journal of Reproduction and Development |
Volume: | 58 |
Issue: | 2 |
Start Page: | 175 |
End Page: | 179 |
Publisher DOI: | 10.1262/jrd.2011-053 |
Abstract: | We studied the longevity of mice produced without sperm using the genomes of oocytes that are already committed to a germline cell lineage. The first sperm-free mouse “KAGUYA”, which we term ‘bi-maternal mouse’, was born on 3 February, 2003. Bi-maternal embryos were generated using 2 sets of female genomes—one derived from fully grown oocytes from normal adults and the other from non-growing oocytes from newborn pups. These genomes were combined by nuclear transfer. We refined the technique for generating bi-maternal mice and found that genetic manipulations in only 2 regions―the imprinting centres of Igf2-H19 and Dlk1-Gtl2―on chromosomes 7 and 12 of the newborn pups allowed us to generate bi-maternal mice at a high rate. Studying bi-maternal conceptuses and mice provides further insight into the mechanisms by which paternally methylated imprinted genes regulate mammalian ontogenesis. |
Description: | SRD Young Investigator Award 2011 |
Type: | article |
URI: | http://hdl.handle.net/2115/68132 |
Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 川原 学
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