| Title: | Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs |
| Authors: | Ogawa, Kosuke Browse this author |
| Miyaji, Hirofumi Browse this author →KAKEN DB |
| Kato, Akihito Browse this author →KAKEN DB |
| Kosen, Yuta Browse this author |
| Momose, Takehito Browse this author |
| Yoshida, Takashi Browse this author |
| Nishida, Erika Browse this author |
| Miyata, Saori Browse this author |
| Murakami, Shusuke Browse this author |
| Takita, Hiroko Browse this author →KAKEN DB |
| Fugetsu, Bunshi Browse this author →KAKEN DB |
| Sugaya, Tsutomu Browse this author →KAKEN DB |
| Kawanami, Masamitsu Browse this author →KAKEN DB |
| Keywords: | Periodontal tissue engineering |
| Fibroblast growth factor-2 (FGF-2) |
| β-tricalcium phosphate (β-TCP) |
| Nanoparticle |
| Biomaterial |
| Issue Date: | Dec-2016 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Journal of Periodontal Research |
| Volume: | 51 |
| Issue: | 6 |
| Start Page: | 758 |
| End Page: | 767 |
| Publisher DOI: | 10.1111/jre.12352 |
| PMID: | 27870141 |
| Abstract: | Background and Objective:
Nanoparticle bioceramics are being investigated for biomedical applications. We fabricated a regenerative scaffold comprising type I collagen and beta-tricalcium phosphate (β-TCP) nanoparticles. Fibroblast growth factor-2 (FGF-2) is a bioeffective signaling molecule that stimulates cell proliferation and wound healing. This study examined the effects on bioactivity from a nano-β-TCP/collagen scaffold loaded with FGF-2, particularly on periodontal tissue wound healing.
Material and Methods:
β-TCP was pulverized into nano-sized particles (84 nm) and was then dispersed. Nano-β-TCP scaffold was prepared by coating the surface of a collagen scaffold with a nano-sized β-TCP dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), compressive testing, cell seeding, and rat subcutaneous implant testing. Then nano-β-TCP scaffold, nano-β-TCP scaffold loaded with FGF-2, and non-coated collagen scaffold were implanted into a dog 1-wall infrabony defect model. Histological observations were made at 10 d and 4 wk post-surgery.
Results:
Results of SEM observation show that TCP nanoparticles were attached to collagen fibers. Nano-β-TCP scaffold showed higher compressive strength and cytocompatibility than non-coated collagen scaffold. Rat subcutaneous implant tests showed that DNA contents of infiltrating cells in the nano-β-TCP scaffold and FGF2-loaded scaffold were each approximately 2.8-fold and 3.7-fold greater than the collagen scaffold. Histological samples from the periodontal defect model showed about five-fold greater periodontal tissue repair following implantation of the nano-β-TCP scaffold loaded with FGF-2 rather than the collagen scaffold.
Conclusion:
The β-TCP nanoparticle coating strongly improved the collagen scaffold bioactivity. Nano-β-TCP scaffolds with FGF-2 are anticipated for use in periodontal tissue engineering. |
| Rights: | This is the peer reviewed version of the following article: [Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs], which has been published in final form at [https://doi.org/10.1111/jre.12352]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/68175 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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