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Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs

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Title: Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs
Authors: Ogawa, Kosuke Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Kato, Akihito Browse this author →KAKEN DB
Kosen, Yuta Browse this author
Momose, Takehito Browse this author
Yoshida, Takashi Browse this author
Nishida, Erika Browse this author
Miyata, Saori Browse this author
Murakami, Shusuke Browse this author
Takita, Hiroko Browse this author →KAKEN DB
Fugetsu, Bunshi Browse this author →KAKEN DB
Sugaya, Tsutomu Browse this author →KAKEN DB
Kawanami, Masamitsu Browse this author →KAKEN DB
Keywords: Periodontal tissue engineering
Fibroblast growth factor-2 (FGF-2)
β-tricalcium phosphate (β-TCP)
Issue Date: Dec-2016
Publisher: Wiley-Blackwell
Journal Title: Journal of Periodontal Research
Volume: 51
Issue: 6
Start Page: 758
End Page: 767
Publisher DOI: 10.1111/jre.12352
PMID: 27870141
Abstract: Background and Objective: Nanoparticle bioceramics are being investigated for biomedical applications. We fabricated a regenerative scaffold comprising type I collagen and beta-tricalcium phosphate (β-TCP) nanoparticles. Fibroblast growth factor-2 (FGF-2) is a bioeffective signaling molecule that stimulates cell proliferation and wound healing. This study examined the effects on bioactivity from a nano-β-TCP/collagen scaffold loaded with FGF-2, particularly on periodontal tissue wound healing. Material and Methods: β-TCP was pulverized into nano-sized particles (84 nm) and was then dispersed. Nano-β-TCP scaffold was prepared by coating the surface of a collagen scaffold with a nano-sized β-TCP dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), compressive testing, cell seeding, and rat subcutaneous implant testing. Then nano-β-TCP scaffold, nano-β-TCP scaffold loaded with FGF-2, and non-coated collagen scaffold were implanted into a dog 1-wall infrabony defect model. Histological observations were made at 10 d and 4 wk post-surgery. Results: Results of SEM observation show that TCP nanoparticles were attached to collagen fibers. Nano-β-TCP scaffold showed higher compressive strength and cytocompatibility than non-coated collagen scaffold. Rat subcutaneous implant tests showed that DNA contents of infiltrating cells in the nano-β-TCP scaffold and FGF2-loaded scaffold were each approximately 2.8-fold and 3.7-fold greater than the collagen scaffold. Histological samples from the periodontal defect model showed about five-fold greater periodontal tissue repair following implantation of the nano-β-TCP scaffold loaded with FGF-2 rather than the collagen scaffold. Conclusion: The β-TCP nanoparticle coating strongly improved the collagen scaffold bioactivity. Nano-β-TCP scaffolds with FGF-2 are anticipated for use in periodontal tissue engineering.
Rights: This is the peer reviewed version of the following article: [Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs], which has been published in final form at []. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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