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Rapid quantitative detection of Salmonella enterica using fluorescence in situ hybridization with filter-cultivation (FISHFC) method

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Title: Rapid quantitative detection of Salmonella enterica using fluorescence in situ hybridization with filter-cultivation (FISHFC) method
Other Titles: 培養併用FISH(FISHFC)法を用いたサルモネラの迅速定量検出
Authors: Shimizu, Shigemasa1 Browse this author
Aoi, Ryouhei2 Browse this author
Osanai, Yui3 Browse this author
Kawai, Yuji4 Browse this author →KAKEN DB
Yamazaki, Koji5 Browse this author →KAKEN DB
Authors(alt): 清水, 茂雅1
青井, 良平2
小山内, 唯3
川合, 祐史4
山崎, 浩司5
Keywords: Salmonella enterica
Fluorescence in situ hybridization
Rapid detection
Issue Date: Jan-2013
Publisher: Japanese Society for Food Science and Technology
Journal Title: Food science and technology research
Volume: 19
Issue: 1
Start Page: 59
End Page: 67
Publisher DOI: 10.3136/fstr.19.59
Abstract: Specific detection and enumeration of Salmonella enterica in food using conventional culture based methods (CCBM) are time-consuming and labor-intensive. This study was done to develop a rapid S. enterica detection and enumeration method that combined fluorescence in situ hybridization (FISH) with micro-colony formation culture, called as FISHFC. The specificity tests of SAL343 probe for S. enterica detection revealed the SAL343-associated fluorescent micro-colonies were observed specifically for S. enterica but not for any other organisms. This finding suggested SAL343 probe is highly specific for detecting S. enterica with FISHFC. For validation, the FISHFC with SAL343-probe were compared with CCBM with multiple selective agar in spiked food samples and there was no significant differences in enumeration was found between FISHFC and CCBMs (p>0.05). The FISHFC methods was able to enumerate S. enterica counts within 10 h and CCBMs takes 5 days to enumerate S. enterica. Therefore, FISHFC methods could be useful in rapid, specific and enumerating S. enterica in food samples compared to other available methods.
従来のサルモネラ検査法は,検査結果を得るまでに時間および労力を必要とする。本研究では,FISHFC 法と呼ばれる検出法を用いたサルモネラの迅速定量検出法を開発することを目的とした。Salmonella enterica検出のためのプローブSAL343の特異性は,S. enterica のみで明瞭な蛍光コロニーを得ることができ,検出特異性は極めて高かった。汚染食品モデルを用いてSAL343プローブを用いたFISHFC法と選択寒天平板培地による培養法でのサルモネラ検出量を比較した結果,平板培地による検出量とFISHFC 法での検出量との間には統計学的な有意差は認められなかった(p>0.05)。また,本研究で開発したFISHFC法では,培養法で約5日間必要であった試料調製から最終結果が得られるまでの時間が10 時間以内に短縮された。よって,FISHFC 法は食品からサルモネラを精度よく迅速定量するための技術であることが実証された。
Type: article
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山﨑 浩司

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