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Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum)

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/68273

Title: Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum)
Authors: Ogawa, Hirohito Browse this author
Kajihara, Masahiro Browse this author
Nao, Naganori Browse this author
Shigeno, Asako Browse this author
Fujikura, Daisuke Browse this author →KAKEN DB
Hang'ombe, Bernard M. Browse this author
Mweene, Aaron S. Browse this author
Mutemwa, Alisheke Browse this author
Squarre, David Browse this author
Yamada, Masao Browse this author
Higashi, Hideaki Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Takada, Ayato Browse this author →KAKEN DB
Keywords: adenovirus
bat
Eidolon helvum
Zambia
Issue Date: Dec-2017
Publisher: MDPI
Journal Title: Viruses
Volume: 9
Issue: 12
Start Page: 371
Publisher DOI: 10.3390/v9120371
Abstract: Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name “Bat mastadenovirus H”. Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/68273
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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