| Title: | Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum) |
| Authors: | Ogawa, Hirohito Browse this author |
| Kajihara, Masahiro Browse this author |
| Nao, Naganori Browse this author |
| Shigeno, Asako Browse this author |
| Fujikura, Daisuke Browse this author →KAKEN DB |
| Hang'ombe, Bernard M. Browse this author |
| Mweene, Aaron S. Browse this author |
| Mutemwa, Alisheke Browse this author |
| Squarre, David Browse this author |
| Yamada, Masao Browse this author |
| Higashi, Hideaki Browse this author →KAKEN DB |
| Sawa, Hirofumi Browse this author →KAKEN DB |
| Takada, Ayato Browse this author →KAKEN DB |
| Keywords: | adenovirus |
| bat |
| Eidolon helvum |
| Zambia |
| Issue Date: | Dec-2017 |
| Publisher: | MDPI |
| Journal Title: | Viruses |
| Volume: | 9 |
| Issue: | 12 |
| Start Page: | 371 |
| Publisher DOI: | 10.3390/v9120371 |
| Abstract: | Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name “Bat mastadenovirus H”. Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/68273 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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