HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

Establishment and characterization of immortalized bovine endometrial epithelial cells

This item is licensed under: Creative Commons Attribution-NonCommercial 3.0 Unported

Files in This Item:
Bai_et_al-2014-Animal_Science_Journal.pdf461.59 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/68346

Title: Establishment and characterization of immortalized bovine endometrial epithelial cells
Authors: Bai, Hanako Browse this author
Sakurai, Toshihiro Browse this author
Bai, Rulan Browse this author
Yamakoshi, Sachiko Browse this author
Aoki, Etsunari Browse this author
Kuse, Mariko Browse this author
Okuda, Kiyoshi Browse this author
Imakawa, Kazuhiko Browse this author
Keywords: bovine
endometrial epithelial cell
immortalization
Issue Date: Aug-2014
Journal Title: Animal Science Journal
Volume: 85
Issue: 8
Start Page: 799
End Page: 804
Publisher DOI: 10.1111/asj.12202
Abstract: Abstract:Bovine primary uterine endometrial epithelial cells (EECs) are not ideal for long-term studies, because primary EECs lose hormone responsiveness quickly, and/or they tend to have a short life span. The aims of this study were to establish immortalized bovine EECs and to characterize these cells following long-term cultures. Immortalized bovine EECs were established by transfecting retroviral vectors encoding human papillomavirus (HPV) E6 and E7, and human telomerase reverse transcriptase (hTERT) genes. Established bovine immortalized EECs (imEECs) showed the same morphology as primary EECs, and could be grown without any apparent changes for over 60 passages. In addition, imEECs have maintained the features as EECs, exhibiting oxytocin (OT) and interferon tau (IFNT) responsiveness. Therefore, these imEECs, even after numbers of passages, could be used as an in vitro model to investigate cellular and molecular mechanisms, by which the uterine epithelium responds to IFNT stimulation, the event required for the maternal recognition of pregnancy in the bovine species.
Rights: © 2014 The Authors. Animal Science Journal published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Society of Animal Science.,This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
https://creativecommons.org/licenses/by-nc/3.0/
Type: article
URI: http://hdl.handle.net/2115/68346
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 唄 花子

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 

 - Hokkaido University