HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

Expression and Potential Role of GATA6 in Ruminant Trophoblasts during Peri-Implantation Periods

Files in This Item:
29_135-1.pdf553.55 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/68352

Title: Expression and Potential Role of GATA6 in Ruminant Trophoblasts during Peri-Implantation Periods
Authors: Bai, Hanako Browse this author
Sakurai, Toshihiro Browse this author
Ideta, Atsushi Browse this author
Aoyagi, Yoshito Browse this author
Godkin, James D. Browse this author
Imakawa, Kazuhiko Browse this author
Keywords: GATA6
Trophoblast cells
Implantation
Ruminants
Issue Date: 2012
Publisher: 日本卵子学会
Journal Title: Journal of Mammalian Ova Research
Volume: 29
Issue: 3
Start Page: 135
End Page: 141
Publisher DOI: 10.1274/jmor.29.135
Abstract: Abstract: Expression of GATA6 has been found during embryonic development in many mammalian species. In mouse embryonic development, GATA6 is a primitive endoderm (PrE) marker. However, the expression and effect of GATA6 in ruminant ungulates has not been well characterized. In this report, the expression of GATA6 mRNA was examined in the uteri of ruminants. GATA6 mRNA was detected in days 17, 20, and 22 (day 0=day of estrus) bovine conceptuses and in days 15, 17, and 21 ovine conceptuses. In both cases, GATA6 mRNA increased on day 22 or 21 after conceptus attachment to the uterine epithelium. GATA6 mRNA was also detected in bovine trophoblast CT-1 or F3 cells and ovine trophoblast oTr cells. In transient transfection analyses using the upstream region of the bovine IFNT gene (bIFNT, IFN-tau-c1), GATA6 overexpression was effective in the up-regulation of the bIFNT construct that had been transfected into human choriocarcinoma JEG3 or bovine ear-derived fibroblast EF cells. The observation in which GATA6 increased IFNT transcription suggests that in addition to lineage specification, GATA6 may have acquired ruminant-specific functions involving the control of trophoblast gene expression.
Rights: © 2012 Japanese Society of Mammalian Ova Research
Type: article
URI: http://hdl.handle.net/2115/68352
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 唄 花子

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 

 - Hokkaido University