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Inhibition of neuropeptide Y Y1 receptor induces osteoblast differentiation in MC3T3-E1 cells

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Title: Inhibition of neuropeptide Y Y1 receptor induces osteoblast differentiation in MC3T3-E1 cells
Authors: Yahara, Motoki Browse this author
Tei, Kanchu Browse this author →KAKEN DB
Tamura, Masato Browse this author →KAKEN DB
Keywords: neuropeptide Y
osteoblastic differentiation
Y1 receptor
Issue Date: Sep-2017
Publisher: Spandidos Publications
Journal Title: Molecular medicine reports
Volume: 16
Issue: 3
Start Page: 2779
End Page: 2784
Publisher DOI: 10.3892/mmr.2017.6866
PMID: 28656295
Abstract: Neuropeptide Y (NPY) is a major neural signaling molecule. NPY is produced by peripheral tissues, such as osteoblasts, and binds to the corresponding Y1 receptor that belongs to the G-protein-coupled receptor family. Osteoblast-specific Y1 receptor knockout mice exhibit high bone mass, indicating a role of the NPY-Y1 receptor axis in the regulation of bone homeostasis. In the bone microenvironment, peripheral nerve fibers and osteoblasts produce NPY. However, the effects of the Y1 receptor on osteoblasts remain unexplored. In the present study, an RNA interference approach was employed to target the Y1 receptor, in order to determine whether it may function to regulate the growth, differentiation and viability of osteoblasts. Knockdown of the Y1 receptor by small interfering RNA (siRNA) lead to induction of alkaline phosphatase (ALP) activity and mineralization in mouse MC3T3-E1 osteoblast cells. In addition, the mRNA expression levels of ALP, osteocalcin, collagen (I) alpha 1, and bone sialoprotein were significantly increased following transfection of a Y1 receptor siRNA. Furthermore, the mRNA expression levels of Runx2 and osterix were significantly increased; however, no significant alterations in cell proliferation and caspase-3/7 activity were observed in Y1 receptor siRNA-transfected cells when compared with non-targeting controls. The results demonstrate that Y1 receptor inhibition may increase osteoblastic differentiation, which indicates a role of the Y1 receptor in the regulation of osteoblastic differentiation.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田村 正人

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