HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
国際連携研究教育局 : GI-CoRE >
雑誌発表論文等  >

In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection

この資料はクリエイティブ・コモンズ・ライセンスの下で公開されています。

フルテキスト
journal.pone.0174916.pdf1.53 MBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/68485

タイトル: In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection
著者: Nishimori, Asami 著作を一覧する
Konnai, Satoru 著作を一覧する
Okagawa, Tomohiro 著作を一覧する
Maekawa, Naoya 著作を一覧する
Ikebuchi, Ryoyo 著作を一覧する
Goto, Shinya 著作を一覧する
Sajiki, Yamato 著作を一覧する
Suzuki, Yasuhiko 著作を一覧する
Kohara, Junko 著作を一覧する
Ogasawara, Satoshi 著作を一覧する
Kato, Yukinari 著作を一覧する
Murata, Shiro 著作を一覧する
Ohashi, Kazuhiko 著作を一覧する
発行日: 2017年 4月26日
出版者: PLOS
誌名: PLoS One
巻: 12
号: 4
開始ページ: e0174916
出版社 DOI: 10.1371/journal.pone.0174916
抄録: Programmed death-1 (PD-1), an immunoinhibitory receptor on T cells, is known to be involved in immune evasion through its binding to PD-ligand 1 (PD-L1) in many chronic diseases. We previously found that PD-L1 expression was upregulated in cattle infected with bovine leukemia virus (BLV) and that an antibody that blocked the PD-1/PD-L1 interaction reactivated Tcell function in vitro. Therefore, this study assessed its antivirus activities in vivo. First, we inoculated the anti-bovine PD-L1 rat monoclonal antibody 4G12 into a BLV-infected cow. However, this did not induce T-cell proliferation or reduction of BLV provirus loads during the test period, and only bound to circulating IgM+ B cells until one week post-inoculation. We hypothesized that this lack of in vivo effects was due to its lower stability in cattle and so established an anti-PD-L1 rat-bovine chimeric antibody (Boch4G12). Boch4G12 was able to bind specifically with bovine PD-L1, interrupt the PD-1/PD-L1 interaction, and activate the immune response in both healthy and BLV-infected cattle in vitro. Therefore, we experimentally infected a healthy calf with BLV and inoculated it intravenously with 1 mg/kg of Boch4G12 once it reached the aleukemic (AL) stage. Cultivation of peripheral blood mononuclear cells (PBMCs) isolated from the tested calf indicated that the proliferation of CD4+ T cells was increased by Boch4G12 inoculation, while BLV provirus loads were significantly reduced, clearly demonstrating that this treatment induced antivirus activities. Therefore, further studies using a large number of animals are required to support its efficacy for clinical application.
資料タイプ: article
URI: http://hdl.handle.net/2115/68485
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 鈴木 定彦

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学