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Ebola virus requires a host scramblase for externalization of phosphatidylserine on the surface of viral particles


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タイトル: Ebola virus requires a host scramblase for externalization of phosphatidylserine on the surface of viral particles
著者: Nanbo, Asuka 著作を一覧する
Maruyama, Junki 著作を一覧する
Imai, Masaki 著作を一覧する
Ujie, Michiko 著作を一覧する
Fujioka, Yoichiro 著作を一覧する
Nishide, Shinya 著作を一覧する
Takada, Ayato 著作を一覧する
Ohba, Yusuke 著作を一覧する
Kawaoka, Yoshihiro 著作を一覧する
発行日: 2018年 1月
出版者: PLOS
誌名: PLOS pathogens
巻: 14
号: 1
開始ページ: e1006848
出版社 DOI: 10.1371/journal.ppat.1006848
抄録: Cell surface receptors for phosphatidylserine contribute to the entry of Ebola virus (EBOV) particles, indicating that the presence of phosphatidylserine in the envelope of EBOV is important for the internalization of EBOV particles. Phosphatidylserine is typically distributed in the inner layer of the plasma membrane in normal cells. Progeny virions bud from the plasma membrane of infected cells, suggesting that phosphatidylserine is likely flipped to the outer leaflet of the plasma membrane in infected cells for EBOV virions to acquire it. Currently, the intracellular dynamics of phosphatidylserine during EBOV infection are poorly understood. Here, we explored the role of XK-related protein (Xkr) 8, which is a scramblase responsible for exposure of phosphatidylserine in the plasma membrane of apoptotic cells, to understand its significance in phosphatidylserine-dependent entry of EBOV. We found that Xkr8 and transiently expressed EBOV glycoprotein GP often co-localized in intracellular vesicles and the plasma membrane. We also found that co-expression of GP and viral major matrix protein VP40 promoted incorporation of Xkr8 into ebolavirus-like particles (VLPs) and exposure of phosphatidylserine on their surface, although only a limited amount of phosphatidylserine was exposed on the surface of the cells expressing GP and/or VP40. Downregulating Xkr8 or blocking caspase-mediated Xkr8 activation did not affect VLP production, but they reduced the amount of phosphatidylserine on the VLPs and their uptake in recipient cells. Taken together, our findings indicate that Xkr8 is trafficked to budding sites via GP-containing vesicles, is incorporated into VLPs, and then promote the entry of the released EBOV to cells in a phosphatidylserine-dependent manner.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 南保 明日香


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