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Identification of Jasmonic Acid and Jasmonoyl-Isoleucine, and Characterization of AOS, AOC, OPR and JAR1 in the Model Lycophyte Selaginella moellendorffii

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Title: Identification of Jasmonic Acid and Jasmonoyl-Isoleucine, and Characterization of AOS, AOC, OPR and JAR1 in the Model Lycophyte Selaginella moellendorffii
Authors: Pratiwi, Putri Browse this author
Tanaka, Genta Browse this author
Takahashi, Tomohiro Browse this author
Xie, Xiaonan Browse this author
Yoneyama, Koichi Browse this author
Matsuura, Hideyuki Browse this author →KAKEN DB
Takahashi, Kosaku Browse this author →KAKEN DB
Keywords: AOC
AOS
JAR1
Jasmonic acid
OPR3
Selaginella moellendorffii
Issue Date: Apr-2017
Publisher: Oxford University Press
Journal Title: Plant and Cell Physiology
Volume: 58
Issue: 4
Start Page: 789
End Page: 801
Publisher DOI: 10.1093/pcp/pcx031
PMID: 28340155
Abstract: Jasmonic acid (JA) is involved in a variety of physiological responses in seed plants. However, the detection and role of JA in lycophytes, a group of seedless vascular plants, have remained elusive until recently. This study provides the first evidence of 12-oxo-phytodienoic acid (OPDA), JA and jasmonoyl-isoleucine (JA-Ile) in the model lycophyte Selaginella moellendorffii. Mechanical wounding stimulated the accumulation of OPDA, JA and JA-Ile. These data were corroborated by the detection of enzymatically active allene oxide synthase (AOS), allene oxide cyclase (AOC), 12-oxo-phytodienoic acid reductase 3 (OPR3) and JA-Ile synthase (JAR1) in S. moellendorffii. SmAOS2 is involved in the first committed step of JA biosynthesis. SmAOC1 is a crucial enzyme for generating the basic structure of jasmonates and is actively involved in the formation of OPDA. SmOPR5, a functionally active OPR3-like enzyme, is also vital for the reduction of (+)-cis-OPDA, the only isomer of the JA precursor. The conjugation of JA to Ile by SmJAR1 demonstrates that S. moellendorffii produces JA-Ile. Thus, the four active enzymes have characteristics similar to those in seed plants. Wounding and JA treatment induced the expression of SmAOC1 and SmOPR5. Furthermore, JA inhibited the growth of shoots in S. moellendorffii, which suggests that JA functions as a signaling molecule in S. moellendorffii. This study proposes that JA evolved as a plant hormone for stress adaptation, beginning with the emergence of vascular plants.
Rights: This is a pre-copyedited, author-produced PDF of an article accepted for publication in Plant & cell physiology following peer review. The version of record 58(4), pp789-801 is available online at: https://doi.org/10.1093/pcp/pcx031
Type: article (author version)
URI: http://hdl.handle.net/2115/68680
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 公咲

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