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Behavioral assay and chemical characters of female sex pheromones in the hermit crab Pagurus filholi

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Title: Behavioral assay and chemical characters of female sex pheromones in the hermit crab Pagurus filholi
Authors: Okamura, Saori Browse this author
Kawaminami, Takuma Browse this author
Matsuura, Hiroshi Browse this author
Fusetani, Nobuhiro Browse this author
Goshima, Seiji Browse this author →KAKEN DB
Keywords: Bioassay
Hermit crab
Low polar molecule
Pagurus filholi
Sex pheromone
Issue Date: May-2017
Publisher: Springer
Journal Title: Journal of ethology
Volume: 35
Issue: 2
Start Page: 169
End Page: 176
Publisher DOI: 10.1007/s10164-017-0507-y
Abstract: Males of the hermit crab Pagurus filholi perform assessment behavior toward females, as a preliminary step of precopulatory guarding, during the reproductive season. It is known that such behavior is elicited by female sex pheromones, but the compounds involved have never been characterized in this species. Several experiments were conducted to develop a reliable bioassay along with purification procedures to identify potential compounds with pheromonal activity in Pagurus filholi. We developed a bioassay protocol to assess pheromonal activity by using an empty shell with cotton containing either artificial seawater (control) or test water. We measured and compared the time duration of male assessment behavior toward each shell if the test water contained female sex pheromones. Ultra-filtering of seawater samples potentially containing pheromones showed that the compound was < 1 kDa in molecular weight. Males showed precopulatory assessment behavior toward "female conditioned" water samples treated with open column purification and eluted with MeOH, suggesting that compounds triggering male behavior were low polar molecules. Molecules with pheromonal activity were not volatile after freeze drying, effective even after heating to 90 A degrees C, and remained active in seawater at 12 A degrees C even after 6 days from sample collection, which suggests a rather stable characteristic of the female sex pheromones of this species.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 五嶋 聖治

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