HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Institute for Genetic Medicine >
Peer-reviewed Journal Articles, etc >

Ecrg4 peptide is the ligand of multiple scavenger receptors

This item is licensed under:Creative Commons Attribution 4.0 International

Files in This Item:
41598_2018_22440_MOESM1_ESM.pdfElectronic supplementary material2.18 MBPDFView/Open
s41598-018-22440-4.pdf1.56 MBPDFView/Open
Please use this identifier to cite or link to this item:

Title: Ecrg4 peptide is the ligand of multiple scavenger receptors
Authors: Moriguchi, Tetsuo Browse this author →KAKEN DB
Takeda, Shuji Browse this author
Iwashita, Shinzo Browse this author
Enomoto, Kei Browse this author
Sawamura, Tatsuya Browse this author
Koshimizu, Uichi Browse this author
Kondo, Toru Browse this author →KAKEN DB
Issue Date: 6-Mar-2018
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 8
Start Page: 4048
Publisher DOI: 10.1038/s41598-018-22440-4
Abstract: Esophageal cancer-related gene 4 (Ecrg4) encodes a hormone-like peptide that is believed to be involved in a variety of physiological phenomena, including tumour suppression. Recent progress in the study of Ecrg4 has shown that Ecrg4 is a proinflammatory factor and induces the expression of several cytokines and chemokines in macrophages/microglia. However, the detailed molecular mechanisms of Ecrg4 signalling, especially the Ecrg4 receptors, remain poorly understood. Here, using retrovirus-mediated expression cloning, we identified lectin-like oxidised low-density lipoprotein receptor-1 (LOX-1) as a membrane protein that binds amino acid residues 71-132 of Ecrg4 (Ecrg4(71-132)). Moreover, in addition to LOX-1, several scavenger receptors, such as Scarf1, Cd36 and Stabilin-1, facilitated the efficient internalisation of Ecrg4(71-132) into cells. A broad competitive inhibitor of scavenger receptors, polyinosinic acid, reduced both the binding of Ecrg4(71-132) and the activation of NF-kappa B in microglia. This activation was dependent on MyD88, an adaptor protein that recruits signalling proteins to Toll-like receptors (TLRs), with the consequent induction of various immune responses. These data suggest that multiple scavenger receptors recognise Ecrg4(71-132) and transduce its signals, together with TLRs, in microglia.
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 近藤 亨

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University