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Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures

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Title: Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures
Authors: He, Qian Browse this author
Okajima, Takaharu Browse this author →KAKEN DB
Onoe, Hiroaki Browse this author
Subagyo, Agus Browse this author
Sueoka, Kazuhisa Browse this author →KAKEN DB
Kuribayashi-Shigetomi, Kaori Browse this author
Issue Date: 14-Mar-2018
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 8
Start Page: 4556
Publisher DOI: 10.1038/s41598-018-22598-x
Abstract: This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development.
Rights: http://creativecommons.org/licenses/by/4.0/.
Type: article
URI: http://hdl.handle.net/2115/70462
Appears in Collections:高等教育推進機構 (Institute for the Advancement of Higher Education) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 繁富 香織

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