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Hokkaido University Collection of Scholarly and Academic Papers >
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Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP-1 expression in 3T3L1 adipocytes
| Title: | Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP-1 expression in 3T3L1 adipocytes |
| Authors: | Abd Eldaim, Mabrouk Attia Browse this author | | Matsuoka, Shinya Browse this author | | Okamatsu-Ogura, Yuko Browse this author →KAKEN DB | | Kamikawa, Akihiro Browse this author | | Ahmed, Mohamed Mohamed Browse this author | | Terao, Akira Browse this author →KAKEN DB | | Nakajima, Kei-ichi Browse this author | | Kimura, Kazuhiro Browse this author →KAKEN DB |
| Issue Date: | Jun-2017 |
| Publisher: | John Wiley & Sons |
| Journal Title: | Genes to cells |
| Volume: | 22 |
| Issue: | 6 |
| Start Page: | 568 |
| End Page: | 582 |
| Publisher DOI: | 10.1111/gtc.12498 |
| PMID: | 28488421 |
| Abstract: | It is well known that retinoic acid (RA) suppresses adipogenesis, although there are some contradicting reports. In this study, we examined the effect of extracellular glucose on RA-induced suppression of adipogenesis in 3T3L1 cell culture. When the cells were cultured in normal glucose medium (NG), the addition of RA suppressed lipid accumulation. However, when cultured in high glucose medium (HG), addition of RA to the cells enhanced lipid accumulation. These changes were accompanied by parallel alterations in fatty acid synthase (FAS) and sterol regulatory element-binding protein (SREBP)-1 gene expression. Transfection of SREBP-1 siRNA suppressed RA-induced enhancement of lipid accumulation and FAS expression in the cells cultured with HG. Transfection of the nuclear form of SREBP-1a cDNA into the cells cultured with NG inhibited RA-induced suppression of lipid accumulation and FAS expression. Moreover, RA- and HG-induced SREBP-1a expression occurred at the early phase of adipogenesis and was dependent on glucocorticoid to induce liver X receptor (LXR) , peroxisomal proliferator-activated receptor (PPAR) and retinoid X receptor (RXR), the key nuclear factors influencing the SREBP-1a gene expression. These results suggest that RA suppresses and enhances lipid accumulation through extracellular glucose concentration-dependent modulation of SREBP-1 expression. |
| Rights: | This is the pre-peer reviewed version of the following article: Mabrouk Attia Abd Eldaim, Shinya Matsuoka, Yuko Okamatsu-Ogura, Akihiro Kamikawa, Mohamed Mohamed Ahmed, Akira Terao, Kei-ichi Nakajima, Kazuhiro Kimura, Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP-1 expression in 3T3L1 adipocytes, Genes to Cells, Vol. 22, Issue 6, Pages 568-582, June 2017, which has been published in final form at https://doi.org/10.1111/gtc.12498 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/70708 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 和弘
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