HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >

Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP-1 expression in 3T3L1 adipocytes

Files in This Item:
GTC.pdf1.04 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/70708

Title: Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP-1 expression in 3T3L1 adipocytes
Authors: Abd Eldaim, Mabrouk Attia Browse this author
Matsuoka, Shinya Browse this author
Okamatsu-Ogura, Yuko Browse this author →KAKEN DB
Kamikawa, Akihiro Browse this author
Ahmed, Mohamed Mohamed Browse this author
Terao, Akira Browse this author →KAKEN DB
Nakajima, Kei-ichi Browse this author
Kimura, Kazuhiro Browse this author →KAKEN DB
Issue Date: Jun-2017
Publisher: John Wiley & Sons
Journal Title: Genes to cells
Volume: 22
Issue: 6
Start Page: 568
End Page: 582
Publisher DOI: 10.1111/gtc.12498
PMID: 28488421
Abstract: It is well known that retinoic acid (RA) suppresses adipogenesis, although there are some contradicting reports. In this study, we examined the effect of extracellular glucose on RA-induced suppression of adipogenesis in 3T3L1 cell culture. When the cells were cultured in normal glucose medium (NG), the addition of RA suppressed lipid accumulation. However, when cultured in high glucose medium (HG), addition of RA to the cells enhanced lipid accumulation. These changes were accompanied by parallel alterations in fatty acid synthase (FAS) and sterol regulatory element-binding protein (SREBP)-1 gene expression. Transfection of SREBP-1 siRNA suppressed RA-induced enhancement of lipid accumulation and FAS expression in the cells cultured with HG. Transfection of the nuclear form of SREBP-1a cDNA into the cells cultured with NG inhibited RA-induced suppression of lipid accumulation and FAS expression. Moreover, RA- and HG-induced SREBP-1a expression occurred at the early phase of adipogenesis and was dependent on glucocorticoid to induce liver X receptor (LXR) , peroxisomal proliferator-activated receptor (PPAR) and retinoid X receptor (RXR), the key nuclear factors influencing the SREBP-1a gene expression. These results suggest that RA suppresses and enhances lipid accumulation through extracellular glucose concentration-dependent modulation of SREBP-1 expression.
Rights: This is the pre-peer reviewed version of the following article: Mabrouk Attia Abd Eldaim, Shinya Matsuoka, Yuko Okamatsu-Ogura, Akihiro Kamikawa, Mohamed Mohamed Ahmed, Akira Terao, Kei-ichi Nakajima, Kazuhiro Kimura, Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP-1 expression in 3T3L1 adipocytes, Genes to Cells, Vol. 22, Issue 6, Pages 568-582, June 2017, which has been published in final form at https://doi.org/10.1111/gtc.12498 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/70708
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 和弘

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University