|
Hokkaido University Collection of Scholarly and Academic Papers >
Research Institute for Electronic Science >
Peer-reviewed Journal Articles, etc >
Super-resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices
This item is licensed under:Creative Commons Attribution-NonCommercial 4.0 International
| Title: | Super-resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices |
| Authors: | Sawada, Kazuaki Browse this author | | Kawakami, Ryosuke Browse this author | | Shigemoto, Ryuichi Browse this author | | Nemoto, Tomomi Browse this author →KAKEN DB |
| Keywords: | dendritic spines | | fixed mouse brain | | prefrontal cortex | | three-dimensional structured illumination microscopy | | tissue clearing methods |
| Issue Date: | May-2018 |
| Publisher: | John Wiley & Sons |
| Journal Title: | European journal of neuroscience |
| Volume: | 47 |
| Issue: | 9 |
| Start Page: | 1033 |
| End Page: | 1042 |
| Publisher DOI: | 10.1111/ejn.13901 |
| Abstract: | Three-dimensional (3D) super-resolution microscopy technique structured illumination microscopy (SIM) imaging of dendritic spines along the dendrite has not been previously performed in fixed tissues, mainly due to deterioration of the stripe pattern of the excitation laser induced by light scattering and optical aberrations. To address this issue and solve these optical problems, we applied a novel clearing reagent, LUCID, to fixed brains. In SIM imaging, the penetration depth and the spatial resolution were improved in LUCID-treated slices, and 160-nm spatial resolution was obtained in a large portion of the imaging volume on a single apical dendrite. Furthermore, in a morphological analysis of spine heads of layer V pyramidal neurons (L5PNs) in the medial prefrontal cortex (mPFC) of chronic dexamethasone (Dex)-treated mice, SIM imaging revealed an altered distribution of spine forms that could not be detected by high-NA confocal imaging. Thus, super-resolution SIM imaging represents a promising high-throughput method for revealing spine morphologies in single dendrites. |
| Rights: | © 2018 Kazuaki Sawada, Ryosuke Kawakami, Ryuichi Shigemoto and Tomomi Nemoto. | | https://creativecommons.org/licenses/by-nc/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/70766 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 根本 知己
|
