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Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke

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Title: Human Recombinant Peptide Sponge Enables Novel, Less Invasive Cell Therapy for Ischemic Stroke
Authors: Miyamoto, Michiyuki Browse this author
Nakamura, Kentaro Browse this author
Shichinohe, Hideo Browse this author →KAKEN DB
Yamauchi, Tomohiro Browse this author
Ito, Masaki Browse this author
Saito, Hisayasu Browse this author
Kawabori, Masahito Browse this author →KAKEN DB
Osanai, Toshiya Browse this author →KAKEN DB
Sasaki, Tasuku Browse this author
Houkin, Kiyohiro Browse this author →KAKEN DB
Kuroda, Satoshi Browse this author →KAKEN DB
Issue Date: 8-Apr-2018
Publisher: Hindawi
Journal Title: Stem Cells International
Volume: 2018
Start Page: 4829534
Publisher DOI: 10.1155/2018/4829534
Abstract: Bone marrow stromal cell (BMSC) transplantation has the therapeutic potential for ischemic stroke. However, it is unclear which delivery routes would yield both safety and maximal therapeutic benefits. We assessed whether a novel recombinant peptide (RCP) sponge, that resembles human collagen, could act as a less invasive and beneficial scaffold in cell therapy for ischemic stroke. BMSCs from green fluorescent protein-transgenic rats were cultured and Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (MCAo). A BMSC-RCP sponge construct was transplanted onto the ipsilateral intact neocortex 7 days after MCAo. A BMSC suspension or vehicle was transplanted into the ipsilateral striatum. Rat motor function was serially evaluated and histological analysis was performed 5 weeks after transplantation. The results showed that BMSCs could proliferate well in the RCP sponge and the BMSC-RCP sponge significantly promoted functional recovery, compared with the vehicle group. Histological analysis revealed that the RCP sponge provoked few inflammatory reactions in the host brain. Moreover, some BMSCs migrated to the peri-infarct area and differentiated into neurons in the BMSC-RCP sponge group. These findings suggest that the RCP sponge may be a promising candidate for animal protein-free scaffolds in cell therapy for ischemic stroke in humans.
Type: article
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川堀 真人

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