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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Listeria monocytogenes serotype 4b strains replicate in monocytes/macrophages more than the other serotypes
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| Title: | Listeria monocytogenes serotype 4b strains replicate in monocytes/macrophages more than the other serotypes |
| Authors: | Hasebe, Rie Browse this author →KAKEN DB | | Nakao, Ryo Browse this author →KAKEN DB | | Ohnuma, Aiko Browse this author | | Yamasaki, Takeshi Browse this author | | Sawa, Hirofumi Browse this author →KAKEN DB | | Takai, Shinji Browse this author →KAKEN DB | | Horiuchi, Motohiro Browse this author →KAKEN DB |
| Keywords: | Listeria monocytogenes | | monocyte/macrophage | | pathogenicity | | serotype |
| Issue Date: | Jun-2017 |
| Publisher: | 公益社団法人 日本獣医学会 |
| Journal Title: | Journal of veterinary medical science |
| Volume: | 79 |
| Issue: | 6 |
| Start Page: | 962 |
| End Page: | 969 |
| Publisher DOI: | 10.1292/jvms.16-0575 |
| Abstract: | We analyzed the pathogenicity of various serotypes of Listeria monocytogenes using a Balb/c mouse intravenous injection model. The survival rates of mice inoculated with strains NS1/2b (serotype 1/2b), NS3b (serotype 3b) and NS 4b (serotype 4b) were 60, 63.6 and 63.6%, respectively. Although the survival rates were similar, the bacterial growth in the liver of NS3b-infected mice was 144.5-fold higher than that in the liver of NS4b-infected mice. Histopathological analyses suggest that the NS4b strain replicated more in monocytes/macrophages, whereas the NS3b strain replicated more in hepatocytes. These results raise a possibility that the serotype 4b strains replicated more in monocytes/macrophages compared to the other serotype strains. To assess this, we isolated CD11b-positive cells from mouse livers infected with EGDe (serotype 1/2a), NS1/2b, NS3b, NS4b and the serotype 4b strains 51414 and F17 and counted the number of live bacteria in these cells. CD11b-positive cells from the NS4b-, 51414- and F17-infected mice possessed 24.4- to 42.7-fold higher numbers of live bacteria than those from mice infected with EGDe and NS3b strains. These results suggest that serotype 4b strains replicated more in monocytes/macrophages than the other serotypes, and this may be involved in the pathogenicity of serotype 4b strains, particularly in the dissemination of L. monocytogenes through the host body. |
| Rights: | © 2017 The Japanese Society of Veterinary Science | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/70785 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 長谷部 理絵
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