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Listeria monocytogenes serotype 4b strains replicate in monocytes/macrophages more than the other serotypes

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Title: Listeria monocytogenes serotype 4b strains replicate in monocytes/macrophages more than the other serotypes
Authors: Hasebe, Rie Browse this author →KAKEN DB
Nakao, Ryo Browse this author →KAKEN DB
Ohnuma, Aiko Browse this author
Yamasaki, Takeshi Browse this author
Sawa, Hirofumi Browse this author →KAKEN DB
Takai, Shinji Browse this author →KAKEN DB
Horiuchi, Motohiro Browse this author →KAKEN DB
Keywords: Listeria monocytogenes
monocyte/macrophage
pathogenicity
serotype
Issue Date: Jun-2017
Publisher: 公益社団法人 日本獣医学会
Journal Title: Journal of veterinary medical science
Volume: 79
Issue: 6
Start Page: 962
End Page: 969
Publisher DOI: 10.1292/jvms.16-0575
Abstract: We analyzed the pathogenicity of various serotypes of Listeria monocytogenes using a Balb/c mouse intravenous injection model. The survival rates of mice inoculated with strains NS1/2b (serotype 1/2b), NS3b (serotype 3b) and NS 4b (serotype 4b) were 60, 63.6 and 63.6%, respectively. Although the survival rates were similar, the bacterial growth in the liver of NS3b-infected mice was 144.5-fold higher than that in the liver of NS4b-infected mice. Histopathological analyses suggest that the NS4b strain replicated more in monocytes/macrophages, whereas the NS3b strain replicated more in hepatocytes. These results raise a possibility that the serotype 4b strains replicated more in monocytes/macrophages compared to the other serotype strains. To assess this, we isolated CD11b-positive cells from mouse livers infected with EGDe (serotype 1/2a), NS1/2b, NS3b, NS4b and the serotype 4b strains 51414 and F17 and counted the number of live bacteria in these cells. CD11b-positive cells from the NS4b-, 51414- and F17-infected mice possessed 24.4- to 42.7-fold higher numbers of live bacteria than those from mice infected with EGDe and NS3b strains. These results suggest that serotype 4b strains replicated more in monocytes/macrophages than the other serotypes, and this may be involved in the pathogenicity of serotype 4b strains, particularly in the dissemination of L. monocytogenes through the host body.
Rights: © 2017 The Japanese Society of Veterinary Science
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/70785
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷部 理絵

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