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Intracerebral, but not intravenous, transplantation of bone marrow stromal cells enhances functional recovery in rat cerebral infarct : An optical imaging study

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Title: Intracerebral, but not intravenous, transplantation of bone marrow stromal cells enhances functional recovery in rat cerebral infarct : An optical imaging study
Other Titles: Direct vs. IV BMSC Delivery
Authors: Kawabori, Masahito Browse this author →KAKEN DB
Kuroda, Satoshi Browse this author →KAKEN DB
Sugiyama, Taku Browse this author
Ito, Masaki Browse this author
Shichinohe, Hideo Browse this author →KAKEN DB
Houkin, Kiyohiro Browse this author →KAKEN DB
Kuge, Yuji Browse this author →KAKEN DB
Tamaki, Nagara Browse this author →KAKEN DB
Keywords: Bone marrow stromal cell
cerebral infarct
cell delivery
transplantation
optical imaging
Issue Date: Jun-2012
Publisher: Wiley
Journal Title: Neuropathology
Volume: 32
Issue: 3
Start Page: 217
End Page: 226
Publisher DOI: 10.1111/j.1440-1789.2011.01260.x
PMID: 22007875
Abstract: Background: Recent studies have indicated that bone marrow stromal cells (BMSC) may improve neurological function when transplanted into animal model of central nervous system (CNS) disorders including cerebral infarct. However, there are few studies that evaluate the therapeutic benefits of direct and intravenous BMSC transplantation for cerebral infarct. Objective: This study was aimed to clarify the favorable route of cell delivery for cerebral infarct in rats. Methods: The rats were subjected to permanent middle cerebral artery occlusion. The BMSC were labeled with near infrared (NIR)-emitting quantum dots, and were transplanted directly (1×10^7 cells) or intravenously (3×10^7 cells) at 7 days after the insult. Using in vivo NIR fluorescence imaging technique, the behaviors of BMSC were serially visualized during 4 weeks after transplantation. Motor function was also assessed. Immunohistochemistry was performed to evaluate the fate of the engrafted BMSC. Results: Direct, but not intravenous, transplantation of BMSC significantly enhanced functional recovery. In vivo NIR fluorescence imaging could clearly visualize their migration towards cerebral infarct during 4 weeks after transplantation in direct group, but not in intravenous, group. The BMSC were widely distributed in the ischemic brain and some of them expressed neural cell markers in the direct group, but not in the intravenous group. Conclusion: These findings strongly suggest that intravenous administration of BMSC has limited effectiveness at clinically relevant timing and direct administration should be chosen for patients with ischemic stroke, although further studies would be warranted to establish the treatment protocol.
Rights: This is the peer reviewed version of the following article: Kawabori, M., Kuroda, S., Sugiyama, T., Ito, M., Shichinohe, H., Houkin, K., Kuge, Y. and Tamaki, N. (2012), Intracerebral, but not intravenous, transplantation of bone marrow stromal cells enhances functional recovery in rat cerebral infarct: An optical imaging study. Neuropathology, 32: 217-226., which has been published in final form at https://doi.org/10.1111/j.1440-1789.2011.01260.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Type: article (author version)
URI: http://hdl.handle.net/2115/70795
Appears in Collections:北海道大学病院 (Hokkaido University Hospital) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川堀 真人

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