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Simultaneous determination of primary and secondary d- and l-amino acids by reversed-phase high-performance liquid chromatography using pre-column derivatization with two-step labelling method

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Title: Simultaneous determination of primary and secondary d- and l-amino acids by reversed-phase high-performance liquid chromatography using pre-column derivatization with two-step labelling method
Authors: Yokoyama, Takehiko Browse this author
Tokuda, Masaharu Browse this author
Amano, Masafumi Browse this author
Mikami, Koji Browse this author →KAKEN DB
Keywords: d-amino acid
d-proline
o-phthalaldehyde (OPA)
N-acetyl- l-cysteine (NAC)
1-(9-fluorenyl)ethyl chloroformate (FLEC)
Issue Date: 21-Jun-2017
Publisher: Taylor & Francis
Journal Title: Bioscience, biotechnology, and biochemistry
Volume: 81
Issue: 9
Start Page: 1681
End Page: 1686
Publisher DOI: 10.1080/09168451.2017.1340090
PMID: 28633617
Abstract: This work describes a method for the simultaneous determination of primary d- and l-amino acids and secondary amino acids such as d- and l-proline. In order to remove interferences in the simultaneous determination of primary and secondary amines, the primary amines were derivatized with o-phthalaldehyde/N-acetyl-l-cysteine (OPA/NAC) and subsequently with 1-(9-fluorenyl)ethyl chloroformate (FLEC) for secondary amines, in a pre-column separation derivatization technique. These fluorescent diastereomers of the amino acids were obtained within 3min at room temperature and determined simultaneously by changing wavelengths during analysis in a single eluting run in the high-performance liquid chromatography column. This method, referred to as the two-step labelling method, is effective for the simultaneous determination of d- and l-amino acids.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 21 June 2017, available online: http://www.tandfonline.com/10.1080/09168451.2017.1340090.
Type: article (author version)
URI: http://hdl.handle.net/2115/70798
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三上 浩司

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