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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
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Simultaneous determination of primary and secondary d- and l-amino acids by reversed-phase high-performance liquid chromatography using pre-column derivatization with two-step labelling method
| Title: | Simultaneous determination of primary and secondary d- and l-amino acids by reversed-phase high-performance liquid chromatography using pre-column derivatization with two-step labelling method |
| Authors: | Yokoyama, Takehiko Browse this author | | Tokuda, Masaharu Browse this author | | Amano, Masafumi Browse this author | | Mikami, Koji Browse this author →KAKEN DB |
| Keywords: | d-amino acid | | d-proline | | o-phthalaldehyde (OPA) | | N-acetyl- l-cysteine (NAC) | | 1-(9-fluorenyl)ethyl chloroformate (FLEC) |
| Issue Date: | 21-Jun-2017 |
| Publisher: | Taylor & Francis |
| Journal Title: | Bioscience, biotechnology, and biochemistry |
| Volume: | 81 |
| Issue: | 9 |
| Start Page: | 1681 |
| End Page: | 1686 |
| Publisher DOI: | 10.1080/09168451.2017.1340090 |
| PMID: | 28633617 |
| Abstract: | This work describes a method for the simultaneous determination of primary d- and l-amino acids and secondary amino acids such as d- and l-proline. In order to remove interferences in the simultaneous determination of primary and secondary amines, the primary amines were derivatized with o-phthalaldehyde/N-acetyl-l-cysteine (OPA/NAC) and subsequently with 1-(9-fluorenyl)ethyl chloroformate (FLEC) for secondary amines, in a pre-column separation derivatization technique. These fluorescent diastereomers of the amino acids were obtained within 3min at room temperature and determined simultaneously by changing wavelengths during analysis in a single eluting run in the high-performance liquid chromatography column. This method, referred to as the two-step labelling method, is effective for the simultaneous determination of d- and l-amino acids. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 21 June 2017, available online: http://www.tandfonline.com/10.1080/09168451.2017.1340090. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/70798 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 三上 浩司
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