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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Microwave-assisted Derivatization of Fatty Acids for Its Measurement in Milk Using High-Performance Liquid Chromatography
| Title: | Microwave-assisted Derivatization of Fatty Acids for Its Measurement in Milk Using High-Performance Liquid Chromatography |
| Authors: | Shrestha, Rojeet Browse this author | | Miura, Yusuke Browse this author | | Hirano, Ken-ichi Browse this author | | Chen, Zhen Browse this author | | Okabe, Hiroaki Browse this author | | Chiba, Hitoshi Browse this author →KAKEN DB | | Hui, Shu-Ping Browse this author →KAKEN DB |
| Keywords: | Microwave-assisted saponification | | microwave-assisted derivatization | | fatty acids | | milk fats |
| Issue Date: | May-2018 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical sciences |
| Volume: | 34 |
| Issue: | 5 |
| Start Page: | 575 |
| End Page: | 582 |
| Publisher DOI: | 10.2116/analsci.17P557 |
| PMID: | 29743430 |
| Abstract: | Fatty acid (FA) profiling of milk has important applications in human health and nutrition. Conventional methods for the saponification and derivatization of FA are time-consuming and laborious. We aimed to develop a simple, rapid, and economical method for the determination of FA in milk. We applied a beneficial approach of microwave-assisted saponification (MAS) of milk fats and microwave-assisted derivatization (MAD) of FA to its hydrazides, integrated with HPLC-based analysis. The optimal conditions for MAS and MAD were determined. Microwave irradiation significantly reduced the sample preparation time from 80 min in the conventional method to less than 3 min. We used three internal standards for the measurement of short-, medium- and long-chain FA. The proposed method showed satisfactory analytical sensitivity, recovery and reproducibility. There was a significant correlation in the milk FA concentrations between the proposed and conventional methods. Being quick, economic, and convenient, the proposed method for the milk FA measurement can be substitute for the convention method. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/70942 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 惠 淑萍
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