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Microwave-assisted Derivatization of Fatty Acids for Its Measurement in Milk Using High-Performance Liquid Chromatography

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/70942

Title: Microwave-assisted Derivatization of Fatty Acids for Its Measurement in Milk Using High-Performance Liquid Chromatography
Authors: Shrestha, Rojeet Browse this author
Miura, Yusuke Browse this author
Hirano, Ken-ichi Browse this author
Chen, Zhen Browse this author
Okabe, Hiroaki Browse this author
Chiba, Hitoshi Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Keywords: Microwave-assisted saponification
microwave-assisted derivatization
fatty acids
milk fats
Issue Date: May-2018
Publisher: 日本分析化学会
Journal Title: Analytical sciences
Volume: 34
Issue: 5
Start Page: 575
End Page: 582
Publisher DOI: 10.2116/analsci.17P557
PMID: 29743430
Abstract: Fatty acid (FA) profiling of milk has important applications in human health and nutrition. Conventional methods for the saponification and derivatization of FA are time-consuming and laborious. We aimed to develop a simple, rapid, and economical method for the determination of FA in milk. We applied a beneficial approach of microwave-assisted saponification (MAS) of milk fats and microwave-assisted derivatization (MAD) of FA to its hydrazides, integrated with HPLC-based analysis. The optimal conditions for MAS and MAD were determined. Microwave irradiation significantly reduced the sample preparation time from 80 min in the conventional method to less than 3 min. We used three internal standards for the measurement of short-, medium- and long-chain FA. The proposed method showed satisfactory analytical sensitivity, recovery and reproducibility. There was a significant correlation in the milk FA concentrations between the proposed and conventional methods. Being quick, economic, and convenient, the proposed method for the milk FA measurement can be substitute for the convention method.
Type: article
URI: http://hdl.handle.net/2115/70942
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 惠 淑萍

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