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Microwave-assisted Derivatization of Fatty Acids for Its Measurement in Milk Using High-Performance Liquid Chromatography

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タイトル: Microwave-assisted Derivatization of Fatty Acids for Its Measurement in Milk Using High-Performance Liquid Chromatography
著者: Shrestha, Rojeet 著作を一覧する
Miura, Yusuke 著作を一覧する
Hirano, Ken-ichi 著作を一覧する
Chen, Zhen 著作を一覧する
Okabe, Hiroaki 著作を一覧する
Chiba, Hitoshi 著作を一覧する
Hui, Shu-Ping 著作を一覧する
キーワード: Microwave-assisted saponification
microwave-assisted derivatization
fatty acids
milk fats
発行日: 2018年 5月
出版者: 日本分析化学会(Japan Society for Analytical Chemistry)
誌名: Analytical sciences
巻: 34
号: 5
開始ページ: 575
終了ページ: 582
出版社 DOI: 10.2116/analsci.17P557
抄録: Fatty acid (FA) profiling of milk has important applications in human health and nutrition. Conventional methods for the saponification and derivatization of FA are time-consuming and laborious. We aimed to develop a simple, rapid, and economical method for the determination of FA in milk. We applied a beneficial approach of microwave-assisted saponification (MAS) of milk fats and microwave-assisted derivatization (MAD) of FA to its hydrazides, integrated with HPLC-based analysis. The optimal conditions for MAS and MAD were determined. Microwave irradiation significantly reduced the sample preparation time from 80 min in the conventional method to less than 3 min. We used three internal standards for the measurement of short-, medium- and long-chain FA. The proposed method showed satisfactory analytical sensitivity, recovery and reproducibility. There was a significant correlation in the milk FA concentrations between the proposed and conventional methods. Being quick, economic, and convenient, the proposed method for the milk FA measurement can be substitute for the convention method.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 惠 淑萍


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