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Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71093

Title: Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes
Authors: Abdel-Ghani, Mohammed. A. Browse this author
Sakaguchi, Kenichiro Browse this author
Kanno, Chihiro Browse this author
Yanagawa, Yojiro Browse this author
Katagiri, Seiji Browse this author →KAKEN DB
Nagano, Masashi Browse this author →KAKEN DB
Keywords: IBMX
Oocytes
Pre-IVM duration
Issue Date: 24-May-2018
Publisher: 日本繁殖生物学会
Journal Title: Journal of Reproduction and Development
Publisher DOI: 10.1262/jrd.2018-004
Abstract: We investigated the effects of pre-maturational (pre-IVM) culture on the developmental competence of small-sized bovine oocytes (110 and < 115 µm). Oocytes were cultured with 3-isobutyl-1-methylxanthine (IBMX) for 0, 5, or 10 h and subjected to in vitro maturation, fertilization, and culture. The cleavage rate (73%) of small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (61 and 62%, respectively). The blastocyst rate (16%) of embryos derived from small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (9 and 8%, respectively). In addition, small-sized oocytes with 5 h pre-IVM had a higher mean cell number in blastocysts (134.1 ± 34.8) than those with 0 and 10 h pre-IVM (100.2 ± 17.2 and 107.8 ± 23.7, respectively). In conclusion, the pre-IVM of small-sized oocytes with IBMX for 5 h improved the developmental competence of bovine oocytes, as well as the quality of blastocysts.
Description: Advance Publication
Type: article
URI: http://hdl.handle.net/2115/71093
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 永野 昌志

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