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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes
| Title: | Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes |
| Authors: | Abdel-Ghani, Mohammed. A. Browse this author | | Sakaguchi, Kenichiro Browse this author | | Kanno, Chihiro Browse this author | | Yanagawa, Yojiro Browse this author | | Katagiri, Seiji Browse this author →KAKEN DB | | Nagano, Masashi Browse this author →KAKEN DB |
| Keywords: | IBMX | | Oocytes | | Pre-IVM duration |
| Issue Date: | 24-May-2018 |
| Publisher: | 日本繁殖生物学会 |
| Journal Title: | Journal of Reproduction and Development |
| Volume: | 64 |
| Issue: | 4 |
| Start Page: | 365 |
| End Page: | 369 |
| Publisher DOI: | 10.1262/jrd.2018-004 |
| Abstract: | We investigated the effects of pre-maturational (pre-IVM) culture on the developmental competence of small-sized bovine oocytes (110 and < 115 µm). Oocytes were cultured with 3-isobutyl-1-methylxanthine (IBMX) for 0, 5, or 10 h and subjected to in vitro maturation, fertilization, and culture. The cleavage rate (73%) of small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (61 and 62%, respectively). The blastocyst rate (16%) of embryos derived from small-sized oocytes with 5 h pre-IVM was higher than those with 0 and 10 h pre-IVM (9 and 8%, respectively). In addition, small-sized oocytes with 5 h pre-IVM had a higher mean cell number in blastocysts (134.1 ± 34.8) than those with 0 and 10 h pre-IVM (100.2 ± 17.2 and 107.8 ± 23.7, respectively). In conclusion, the pre-IVM of small-sized oocytes with IBMX for 5 h improved the developmental competence of bovine oocytes, as well as the quality of blastocysts. |
| Description: | Advance Publication |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71093 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永野 昌志
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