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Effect of a single epidural administration of follicle-stimulating hormone via caudal vertebrae on superstimulation for in vivo and in vitro embryo production in Japanese black cows
Title: | Effect of a single epidural administration of follicle-stimulating hormone via caudal vertebrae on superstimulation for in vivo and in vitro embryo production in Japanese black cows |
Authors: | SAKAGUCHI, Kenichiro Browse this author | IDETA, Atsushi Browse this author | YANAGAWA, Yojiro Browse this author | NAGANO, Masashi Browse this author →KAKEN DB | KATAGIRI, Seiji Browse this author | KONISHI, Masato Browse this author |
Keywords: | Epidural administration | Follicle-stimulating hormone (FSH) | Ovum-pick up | Superovulation |
Issue Date: | 16-Jun-2018 |
Publisher: | 日本繁殖生物学会 |
Journal Title: | Journal of Reproduction and Development |
Volume: | 64 |
Issue: | 5 |
Start Page: | 451 |
End Page: | 455 |
Publisher DOI: | 10.1262/jrd.2018-007 |
Abstract: | Here, we describe a simplified procedure for embryo production in the Japanese black cow that uses a single caudal epidural injection of follicle-stimulating hormone (FSH). First, we compared the efficiency of superovulation for in vivo embryo production between conventional multiple FSH treatment (control, n = 10) and single epidural administration (epidural, n = 5). The number of transferable blastocysts was similar between control and epidural groups (4.7 ± 3.5 and 9.0 ± 6.0, respectively). Next, we compared in vitro embryo production by ovum pick-up and in vitro fertilization (OPU-IVF) between control (n = 12) and epidural groups (n = 12). The rate of development to transferable blastocysts was higher in the epidural group than in the control (23.3 vs. 10.5%, P < 0.001). In conclusion, a single epidural administration of FSH can induce follicular development comparable to that of the conventional superovulation protocol and may improve the productivity of OPU-IVF. |
Description: | Advance Publication |
Type: | article |
URI: | http://hdl.handle.net/2115/71095 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 永野 昌志
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