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Molecular identification and characterization of piroplasm species in Hokkaido sika deer (Cervus nippon yesoensis), Japan

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Title: Molecular identification and characterization of piroplasm species in Hokkaido sika deer (Cervus nippon yesoensis), Japan
Authors: Elbaz, Elzahara Browse this author
Moustafa, Mohamed Abdallah Mohamed Browse this author
Lee, Kyunglee Browse this author
Mohamed, Wessam Mohamed Ahmed Browse this author
Nakao, Ryo Browse this author →KAKEN DB
Shimozuru, Michito Browse this author
Sashika, Mariko Browse this author
Younis, Emad Elsayed Ahmed Browse this author
El-Khodery, Sabry Ahmed Browse this author
Tsubota, Toshio Browse this author →KAKEN DB
Keywords: Babesia
Theileria
Sika deer
Reverse line blot
Issue Date: Aug-2017
Publisher: Elsevier
Journal Title: Ticks and Tick-Borne Diseases
Volume: 8
Issue: 5
Start Page: 802
End Page: 807
Publisher DOI: 10.1016/j.ttbdis.2017.06.007
PMID: 28651947
Abstract: Babesia and Theileria species are tick-borne protozoan parasites that have a veterinary and zoonotic importance. In order to investigate the prevalence and genetic diversity of these parasites, a total of 269 sika deer blood DNA samples collected from Hokkaido, Japan, were examined for Babesia and Theileria species by touch-down PCR targeting the 18S rRNA gene. Reverse line blot (RLB) hybridization was then used to detect 12 piroplasm species. The results revealed that 95.5% (257/269), 94.1% (253/269), 14.1% (38/269), 87.7% (236/269) and 11.5% (31/269) of the examined PCR products hybridized with the probes which were designed to detect all Babesia and Theileria spp., all Theileria spp., all Babesia spp., Theileria sp. Thrivae and Babesia divergens-like, respectively. The 18S rRNA gene partial sequences were divided into Theileria sp. Thrivae, T. capreoli, B. divergens-like and an undescribed Babesia species. This study showed the first detection of the undescribed Babesia sp. from Japan. Therefore, more studies are required to understand the ecology of the newly detected tick-borne pathogens in Hokkaido.
Rights: © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/71113
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 坪田 敏男

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