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Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts

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Title: Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts
Authors: Otomo, Kohei Browse this author
Hibi, Terumasa Browse this author
Fang, Yi-Cheng Browse this author
Hung, Jui-Hung Browse this author
Tsutsumi, Motosuke Browse this author
Kawakami, Ryosuke Browse this author
Yokoyama, Hiroyuki Browse this author
Nemoto, Tomomi Browse this author →KAKEN DB
Issue Date: 1-Jun-2018
Publisher: Optical Society of America
Journal Title: Biomedical Optics Express
Volume: 9
Issue: 6
Start Page: 2671
End Page: 2680
Publisher DOI: 10.1364/BOE.9.002671
Abstract: We developed a compact stimulated emission depletion (STED) two-photon excitation microscopy that utilized electrically controllable components. Transmissive liquid crystal devices inserted directly in front of the objective lens converted the STED light into an optical vortex while leaving the excitation light unaffected. Light pulses of two different colors, 1.06 and 0.64 mu m, were generated by laser diode-based light sources, and the delay between the two pulses was flexibly controlled so as to maximize the fluorescence suppression ratio. In our experiments, the spatial resolution of this system was up to three times higher than that obtained without STED light irradiation, and we successfully visualize the fine microtubule network structures in fixed mammalian cells without causing significant photo-damage. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Rights: © 2018 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.
Type: article
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 根本 知己

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