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Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts
| Title: | Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts |
| Authors: | Otomo, Kohei Browse this author | | Hibi, Terumasa Browse this author | | Fang, Yi-Cheng Browse this author | | Hung, Jui-Hung Browse this author | | Tsutsumi, Motosuke Browse this author | | Kawakami, Ryosuke Browse this author | | Yokoyama, Hiroyuki Browse this author | | Nemoto, Tomomi Browse this author →KAKEN DB |
| Issue Date: | 1-Jun-2018 |
| Publisher: | Optical Society of America |
| Journal Title: | Biomedical Optics Express |
| Volume: | 9 |
| Issue: | 6 |
| Start Page: | 2671 |
| End Page: | 2680 |
| Publisher DOI: | 10.1364/BOE.9.002671 |
| Abstract: | We developed a compact stimulated emission depletion (STED) two-photon excitation microscopy that utilized electrically controllable components. Transmissive liquid crystal devices inserted directly in front of the objective lens converted the STED light into an optical vortex while leaving the excitation light unaffected. Light pulses of two different colors, 1.06 and 0.64 mu m, were generated by laser diode-based light sources, and the delay between the two pulses was flexibly controlled so as to maximize the fluorescence suppression ratio. In our experiments, the spatial resolution of this system was up to three times higher than that obtained without STED light irradiation, and we successfully visualize the fine microtubule network structures in fixed mammalian cells without causing significant photo-damage. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
| Rights: | © 2018 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71158 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 根本 知己
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