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Evaluation of acceptor selectivity of Lactococcus lactis ssp. lactis trehalose 6-phosphate phosphorylase in the reverse phosphorolysis and synthesis of a new sugar phosphate

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Title: Evaluation of acceptor selectivity of Lactococcus lactis ssp. lactis trehalose 6-phosphate phosphorylase in the reverse phosphorolysis and synthesis of a new sugar phosphate
Authors: Taguchi, Yodai Browse this author
Saburi, Wataru Browse this author →KAKEN DB
Imai, Ryozo Browse this author
Mori, Haruhide Browse this author →KAKEN DB
Keywords: trehalose 6-phosphate phosphorylase
glycoside hydrolase family 65
reverse phosphorolysis
substrate specificity
Issue Date: Aug-2017
Publisher: Taylor & Francis
Journal Title: Bioscience biotechnology and biochemistry
Volume: 81
Issue: 8
Start Page: 1512
End Page: 1519
Publisher DOI: 10.1080/09168451.2017.1329620
PMID: 28537141
Abstract: Trehalose 6-phosphate phosphorylase (TrePP), a member of glycoside hydrolase family 65, catalyzes the reversible phosphorolysis of trehalose 6-phosphate (Tre6P) with inversion of the anomeric configuration to produce beta-D-glucose 1-phosphate (beta-Glc1P) and D-glucose 6-phosphate (Glc6P). TrePP in Lactococcus lactis ssp. lactis (LlTrePP) is, alongside the phosphotransferase system, involved in the metabolism of trehalose. In this study, recombinant LlTrePP was produced and characterized. It showed its highest reverse phosphorolytic activity at pH 4.8 and 40 degrees C, and was stable in the pH range 5.0-8.0 and at up to 30 degrees C. Kinetic analyses indicated that reverse phosphorolysis of Tre6P proceeded through a sequential bi bi mechanism involving the formation of a ternary complex of the enzyme, beta-Glc1P, and Glc6P. Suitable acceptor substrates were Glc6P, and, at a low level, D-mannose 6-phosphate (Man6P). From beta-Glc1P and Man6P, a novel sugar phosphate, alpha-D-Glcp-(1 <-> 1)-alpha-D-Manp6P, was synthesized with 51% yield.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on Aug 2017, available online: http://www.tandfonline.com/10.1080/09168451.2017.1329620.
Type: article (author version)
URI: http://hdl.handle.net/2115/71175
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田口 陽大

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