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Volume 66 Number 3 >

Expression of miR-29, miR-125, and miR-181 in the anterior kidneys of Streptococcus-infected Nile tilapia (Oreochromis niloticus)

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.66.3.165

Title: Expression of miR-29, miR-125, and miR-181 in the anterior kidneys of Streptococcus-infected Nile tilapia (Oreochromis niloticus)
Authors: Chiachanpongse, Danudej Browse this author
Roongsitthichai, Atthaporn Browse this author
Surachetpong, Win Browse this author
Keywords: microRNAs
Nile tilapia
qPCR
Streptococcus agalactiae
Issue Date: Aug-2018
Publisher: Faculty of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 66
Issue: 3
Start Page: 165
End Page: 176
Abstract: MicroRNAs (miRNAs) are a group of small non-coding RNAs which posttranscriptionally regulate gene expression in eukaryotic cells. In this study, three miRNAs participating in the immune function in mammalian cells: miR-29, miR-125, and miR-181 have been characterized in Nile tilapia (Oreochromis niloticus). A comparison of all three miRNAs in Nile tilapia and other organisms, including zebrafish, mouse, rat, chicken, pig, and human revealed identical sequence of mature miRNA sequences. The genomic sequences of miR-29, miR-125, and miR-181 of Nile tilapia have been amplified using polymerase chain reaction (PCR) and cloned into pGT-19T plasmid. The plasmids containing the sequences of miR-29, miR-125, and miR-181 were used for standardization and validation of quantitative PCR (qPCR) methods. Moreover, the qPCR methods have been applied as a tool to determine the expression of all three miRNAs in the anterior kidneys of Streptococcus agalactiae-infected tilapia. Specifically, expression of miR-29, miR-125, and miR-181 reduced significantly at 3 days post bacterial challenge. In silico analysis of three miRNA targets revealed that multiple genes could be regulated by these miRNAs including Ifi30, Cd276, Lfng, Cdc42se1, Ddx3x, Il1a, and Atg5. In conclusion, these results demonstrated the expression level and molecular targets of miRNAs in Nile tilapia which could be applied for the understanding of these miRNAs during pathogen-host interaction.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/71337
Appears in Collections:Japanese Journal of Veterinary Research > Volume 66 Number 3

Submitter: 獣医学部図書室

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