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Development of nuclear DNA markers to characterize genetically diverse groups of Misgurnus anguillicaudatus and its closely related species

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71424

Title: Development of nuclear DNA markers to characterize genetically diverse groups of Misgurnus anguillicaudatus and its closely related species
Authors: Fujimoto, Takafumi Browse this author →KAKEN DB
Yamada, Aya Browse this author
Kodo, Yukihiro Browse this author
Nakaya, Kohei Browse this author
Okubo-Murata, Michiko Browse this author
Saito, Taiju Browse this author
Ninomiya, Kazuto Browse this author
Inaba, Michiko Browse this author
Kuroda, Masamichi Browse this author
Arai, Katsutoshi Browse this author →KAKEN DB
Murakami, Masaru Browse this author →KAKEN DB
Keywords: Repetitive DNA sequence
RFLP
Hybrid
Species identification
Cobitidae
Issue Date: Sep-2017
Publisher: Springer
Journal Title: Fisheries science
Volume: 83
Issue: 5
Start Page: 743
End Page: 756
Publisher DOI: 10.1007/s12562-017-1108-y
Abstract: Repetitive DNA sequences, ManDra and ManBgl, were isolated from the DraI and BglII digests of the genomic DNA of Misgurnus anguillicaudatus, respectively. A primer set of ManDra distinguished two genetically different groups (A and B) of M. anguillicaudatus by specific electrophoretograms. A primer set of ManBgl amplified the DNA of M. anguillicaudatus and M. mizolepis. The individuals of M. anguillicaudatus were divided into two groups depending on the fragment sizes, in which the groups A and B (B-1 and B-2) showed 400 and 460 bp, respectively. M. mizolepis was distinguished by a different pattern (400-, 460-, and 510-bp fragments). PCR-RFLP analyses of recombination activating gene 1 gave a clear difference between A or B-2 (443-bp fragment) and B-1 groups (296- and 147-bp fragments). Clonal lineages and hybrids between B-1 and B-2 groups could be identified by appearance of three fragments (443, 296, and 147 bp). The combined analyses using the above three nuclear markers discriminated among nuclear genomes of genetic groups (A, B-1 and B-2) of M. anguillicaudatus and M. mizolepis. In several localities, natural hybridizations between the group B-1 and B-2 loaches and introgressions of clonal mitochondrial genomes into the group B-1 loaches were detected.
Rights: The final publication is available at www.springerlink.com via http://dx.doi.org/10.1007/s12562-017-1108-y
Type: article (author version)
URI: http://hdl.handle.net/2115/71424
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藤本 貴史

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