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Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein

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タイトル: Polypentagonal ice-like water networks emerge solely in an activity-improved variant of ice-binding protein
著者: Mahatabuddin, Sheikh 著作を一覧する
Fukami, Daichi 著作を一覧する
Arai, Tatsuya 著作を一覧する
Nishimiya, Yoshiyuki 著作を一覧する
Shimizu, Rumi 著作を一覧する
Shibazaki, Chie 著作を一覧する
Kondo, Hidemasa 著作を一覧する
Adachi, Motoyasu 著作を一覧する
Tsuda, Sakae 著作を一覧する
キーワード: hydration shell
ice-binding protein
crystal structure
water cluster
polypentagonal waters
発行日: 2018年 5月22日
出版者: National Academy of Sciences.
誌名: Proceedings of the National Academy of Sciences of the United States of America (PNAS)
巻: 115
号: 21
開始ページ: 5456
終了ページ: 5461
出版社 DOI: 10.1073/pnas.1800635115
PMID: 29735675
抄録: Polypentagonal water networks were recently observed in a protein capable of binding to ice crystals, or ice-binding protein (IBP). To examine such water networks and clarify their role in ice-binding, we determined X-ray crystal structures of a 65-residue defective isoform of a Zoarcidae-derived IBP (wild type, WT) and its five single mutants (A20L, A20G, A20T, A20V, and A20I). Polypentagonal water networks composed of similar to 50 semiclathrate waters were observed solely on the strongest A20I mutant, which appeared to include a tetrahedral water cluster exhibiting a perfect position match to the (1010) first prism plane of a single ice crystal. Inclusion of another symmetrical water cluster in the poly-pentagonal network showed a perfect complementarity to the waters constructing the (2021) pyramidal ice plane. The order of ice-binding strength was A20L < A20G < WT < A20T < A20V < A20I, where the top three mutants capable of binding to the first prism and the pyramidal ice planes commonly contained a bifurcated gamma-CH3 group. These results suggest that a fine-tuning of the surface of Zoarcidae-derived IBP assisted by a side-chain group regulates the holding property of its polypentagonal water network, the function of which is to freeze the host protein to specific ice planes.
Rights: Copyright (C) 2018 the Author(s). Published by PNAS
資料タイプ: article
URI: http://hdl.handle.net/2115/71437
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 津田 栄

 

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