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siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells
| Title: | siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells |
| Authors: | Tanaka, Hiroki Browse this author | | Shirkoohi, Reza Browse this author | | Nakagawa, Koji Browse this author | | Qiao, Hongjiang Browse this author | | Fujita, Hisakazu Browse this author | | Okada, Futoshi Browse this author | | Hamada, Jun-ichi Browse this author | | Kuzumaki, Satoshi8 Browse this author | | Takimoto, Masato Browse this author | | Kuzumaki, Noboru Browse this author |
| Authors(alt): | 葛巻, 暹8 |
| Keywords: | actin-regulatory protein | | small interfering RNA | | EMT |
| Issue Date: | 10-Oct-2005 |
| Publisher: | Wiley-Liss, Inc. |
| Journal Title: | International Journal of Cancer |
| Volume: | 118 |
| Issue: | 7 |
| Start Page: | 1680 |
| End Page: | 1691 |
| Publisher DOI: | 10.1002/ijc.21559 |
| PMID: | 16217750 |
| Abstract: | Epithelial-mesenchymal transition (EMT) describes a process occurring during development and oncogenesis by which epithelial cells obtain fibroblast-like properties and show reduced cell adhesion and increased motility. In this report we demonstrated typical EMT in human mammary epithelial MCF10A siRNA gelsolin knockdown cells. EMT was characterized by fibroblastic morphology, loss of contact inhibition and focus formation in monolayer growth, enhanced motility and invasiveness in vitro, increased actin filaments, overexpression of RAC, activation of both ERK and AKT, inactivation of GSK3, conversion of cadherin from the E- to N-type, and induction of the transcription factor Snail. These results suggested that gelsolin functions as a switch that controls E- and N-cadherin conversion via Snail,and demonstrated that its knockdown leads to EMT in human mammary epithelial cells and possibly to the development of human mammary tumors. |
| Rights: | Copyright (c) 2005 Wiley-Liss, Inc |
| Relation: | http://www.interscience.wiley.com/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/715 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 葛巻 暹
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