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siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells

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Title: siRNA gelsolin knockdown induces epithelial-mesenchymal transition with a cadherin switch in human mammary epithelial cells
Authors: Tanaka, Hiroki Browse this author
Shirkoohi, Reza Browse this author
Nakagawa, Koji Browse this author
Qiao, Hongjiang Browse this author
Fujita, Hisakazu Browse this author
Okada, Futoshi Browse this author
Hamada, Jun-ichi Browse this author
Kuzumaki, Satoshi8 Browse this author
Takimoto, Masato Browse this author
Kuzumaki, Noboru Browse this author
Authors(alt): 葛巻, 暹8
Keywords: actin-regulatory protein
small interfering RNA
EMT
Issue Date: 10-Oct-2005
Publisher: Wiley-Liss, Inc.
Journal Title: International Journal of Cancer
Volume: 118
Issue: 7
Start Page: 1680
End Page: 1691
Publisher DOI: 10.1002/ijc.21559
PMID: 16217750
Abstract: Epithelial-mesenchymal transition (EMT) describes a process occurring during development and oncogenesis by which epithelial cells obtain fibroblast-like properties and show reduced cell adhesion and increased motility. In this report we demonstrated typical EMT in human mammary epithelial MCF10A siRNA gelsolin knockdown cells. EMT was characterized by fibroblastic morphology, loss of contact inhibition and focus formation in monolayer growth, enhanced motility and invasiveness in vitro, increased actin filaments, overexpression of RAC, activation of both ERK and AKT, inactivation of GSK3, conversion of cadherin from the E- to N-type, and induction of the transcription factor Snail. These results suggested that gelsolin functions as a switch that controls E- and N-cadherin conversion via Snail,and demonstrated that its knockdown leads to EMT in human mammary epithelial cells and possibly to the development of human mammary tumors.
Rights: Copyright (c) 2005 Wiley-Liss, Inc
Relation: http://www.interscience.wiley.com/
Type: article (author version)
URI: http://hdl.handle.net/2115/715
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 葛巻 暹

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