| Title: | Identification and expression analysis of cDNA encoding insulin-like growth factor 2 in horses |
| Authors: | Kikuchi, Kohta Browse this author |
| Sasaki, Keisuke Browse this author |
| Akizawa, Hiroki Browse this author |
| Tsukahara, Hayato Browse this author |
| Bai, Hanako Browse this author |
| Takahashi, Masashi Browse this author →KAKEN DB |
| Nambo, Yasuo Browse this author |
| Hata, Hiroshi Browse this author |
| Kawahara, Manabu Browse this author →KAKEN DB |
| Keywords: | Amino acid sequence |
| Horse |
| Insulin-like growth factor 2 |
| Untranslated region |
| Issue Date: | 27-Feb-2018 |
| Publisher: | Society for Reproduction and Development（SRD） |
| 日本繁殖生物学会 |
| Journal Title: | Journal of Reproduction and Development |
| Volume: | 64 |
| Issue: | 1 |
| Start Page: | 57 |
| End Page: | 64 |
| Publisher DOI: | 10.1262/jrd.2017-124 |
| Abstract: | Insulin-like growth factor 2 (IGF2) is responsible for a broad range of physiological processes during fetal development and adulthood, but genomic analyses of IGF2 containing the 5ʹ- and 3ʹ-untranslated regions (UTRs) in equines have been limited. In this study, we characterized the IGF2 mRNA containing the UTRs, and determined its expression pattern in the fetal tissues of horses. The complete equine IGF2 mRNA sequence harboring another exon approximately 2.8 kb upstream from the canonical transcription start site was identified as a new transcript variant. As this upstream exon did not contain the start codon, the amino acid sequence was identical to the canonical variant. Analysis of the deduced amino acid sequence revealed that the protein possessed two major domains, IlGF and IGF2_C, and analysis of IGF2 sequence polymorphism in fetal tissues of Hokkaido native horse and Thoroughbreds revealed a single nucleotide polymorphism (T to C transition) at position 398 in Thoroughbreds, which caused an amino acid substitution at position 133 in the IGF2 sequence. Furthermore, the expression pattern of the IGF2 mRNA in the fetal tissues of horses was determined for the first time, and was found to be consistent with those of other species. Taken together, these results suggested that the transcriptional and translational products of the IGF2 gene have conserved functions in the fetal development of mammals, including horses. |
| Rights: | ©2018 by the Society for Reproduction and Development., This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
| https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71532 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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