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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Selection and functional analysis of a Pyropia yezoensis ammonium transporter PyAMT1 in potassium deficiency
| Title: | Selection and functional analysis of a Pyropia yezoensis ammonium transporter PyAMT1 in potassium deficiency |
| Authors: | Adams, Eri Browse this author | | Mikami, Koji Browse this author →KAKEN DB | | Shin, Ryoung Browse this author |
| Keywords: | Ammonium transporter | | Full-length cDNA library | | Potassium deficiency | | Pyropia yezoensis | | Salt tolerance |
| Issue Date: | Oct-2017 |
| Journal Title: | Journal of Applied Phycology |
| Volume: | 29 |
| Issue: | 5 |
| Start Page: | 2617 |
| End Page: | 2626 |
| Publisher DOI: | 10.1007/s10811-017-1196-1 |
| Abstract: | Seaweeds are believed to have developed unique mechanisms to maintain optimal cellular potassium and sodium concentrations in order to survive in the saline marine environment. To gain a molecular understanding of underlying potassium/sodium homeostasis in seaweeds, full-length cDNA libraries from the multiple stages in the life cycle, including gametophytes, conchosporangia and sporophytes of a marine red alga, Pyropia yezoensis, were constructed. A large portion of genes from each library through the life cycle was revealed to be functionally unknown reconfirming the uniqueness of P. yezoensis genes in terms of evolutionary lineage. Genes that could potentially contribute to potassium deficiency tolerance were selected from the potassium uptake defective Escherichia coli strain expressing gametophytes and conchosporangia libraries under the low potassium conditions. Of those, an ammonium transporter gene, PyAMT1, was demonstrated to enhance potassium deficiency tolerance effectively when expressed in the E. coli strain. Potential roles of PyAMT1 and other candidate components in this context are discussed. |
| Rights: | This is a post-peer-review, pre-copyedit version of an article published in Journal of Applied Phycology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10811-017-1196-1. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/71573 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 三上 浩司
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