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Isoflavones and their metabolites influence the milk component synthesis ability of mammary epithelial cells through prolactin/STAT5 signaling

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Title: Isoflavones and their metabolites influence the milk component synthesis ability of mammary epithelial cells through prolactin/STAT5 signaling
Authors: Tsugami, Yusaku Browse this author
Matsunaga, Kota Browse this author
Suzuki, Takahiro Browse this author
Nishimura, Takanori Browse this author
Kobayashi, Ken Browse this author →KAKEN DB
Keywords: β-casein
Mammary epithelial cell
Prolactin receptor
Issue Date: Oct-2017
Publisher: John Wiley & Sons
Journal Title: Molecular nutrition & food research
Volume: 61
Issue: 10
Start Page: 1700156
Publisher DOI: 10.1002/mnfr.201700156
PMID: 28605125
Abstract: Scope: Isoflavones are a class of polyphonic compounds present in legumes and are called phytoestrogens because of their estrogen-like activity. Estrogen influences the behavior of mammary epithelial cells (MECs) during pregnancy and lactation. In this study, we investigated the direct influences of isoflavones and their metabolites in milk production ability of MECs. Methods and results: Mouse MECs were cultured with prolactin and dexamethasone (glucocorticoid analog) to induce milk production ability. Subsequently, lactating MECs were treated with each isoflavone. Coumestrol, biochanin A, genistein, and formononetin decreased the intracellular and secreted beta-casein. On the other hand, p-ethylphenol, daidzein, and equol did not significantly influence beta-casein production at any concentration. Coumestrol, biochanin A and genistein down-regulated the mRNA expression of whey acidic protein (WAP), lactoferrin and alpha-lactalbumin. In contrast, p-ethylphenol, daidzein and equol up-regulated beta-casein and/or WAP with alpha-lactalbumin. Furthermore, coumestrol and genistein down-regulated the expression of prolactin receptor and signal transducer and activator of transcription 5 (STAT5) accompanied by a decrease in STAT5 phosphorylation. Conclusion: Isoflavones and their metabolites influence the milk production ability of MECs through different interactions with prolactin/STAT5 signaling. Simultaneous intake of multiple isoflavones by consumption of legumes may induce promotive or adverse effects on lactating MECs.
Rights: This is the peer reviewed version of the following article:Molecular Nutrition & Food Research; 61(10) 1700156; Oct 2017, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 謙

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