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Gelsolin gene silencing involving unusual hypersensitivities to dimethylsulfate and KMnO4 in vivo footprinting on its promoter region
| Title: | Gelsolin gene silencing involving unusual hypersensitivities to dimethylsulfate and KMnO4 in vivo footprinting on its promoter region |
| Other Titles: | Gene silencing of gelsolin in bladder cancer |
| Authors: | Haga, Kazunori Browse this author | | Fujita, Hisakazu Browse this author | | Nomoto, Minoru Browse this author | | Sazawa, Ataru Browse this author | | Nakagawa, Koji Browse this author | | Harabayashi, Toru Browse this author | | Shinohara, Nobuo Browse this author | | Takimoto, Masato Browse this author | | Nonomura, Katsuya Browse this author | | Kuzumaki, Noboru10 Browse this author |
| Authors(alt): | 葛巻, 暹10 |
| Keywords: | gelsolin | | gene silencing | | dimethylsulfate hypersensitivity | | bladder cancer | | in vivo footprinting |
| Issue Date: | 20-May-2004 |
| Publisher: | Wiley-Liss, Inc. |
| Journal Title: | International Journal of Cancer |
| Volume: | 111 |
| Issue: | 6 |
| Start Page: | 873 |
| End Page: | 880 |
| Publisher DOI: | 10.1002/ijc.20348 |
| PMID: | 15300799 |
| Abstract: | We previously reported that gelsolin gene expression is reduced in various tumors. In an effort to gain further insights into the mechanism of gelsolin downregulation in tumors, we examined the in vivo properties of the gelsolin promoter in urinary bladder cancer cell lines. Neither mutation nor hypermethylation were responsible for gene silencing at the promoter. After exposure to trichostatin A (TSA), a histone deacetylase inhibitor, gelsolin promoter activity was markedly enhanced in the cancer cells not in cells derived from normal tissue. Chromatin immunoprecipitation (ChIP) assays revealed that both histones H3 and H4 were hypoacetylated in the promoter region of the cancer cells, and the accumulation of acetylated histones were detected by TSA treatment. In vivo footprinting analysis revealed the presence of dimethylsulfate (DMS) hypersensitive site in the untranslated region around nucleotide -35 only in the cancer cells but not in cells derived from normal tissue, and analysis of KMnO4 reactive nucleotides showed that the stem loop structure could be formed in vivo of the cancer cells. This novel stem loop structure may play a part in regulating the transcription of the gelsolin gene in the cancer cells. .. These results suggest that nucleosome accessibility through histone deacetylation and structural changes (DMS hypersensitivity and stem loop structure) in the promoter region form the basis of the mechanism leading to the silencing of gelsolin gene in human bladder cancer. |
| Rights: | Copyright (c) 2004 Wiley-Liss, Inc |
| Relation: | http://www.interscience.wiley.com/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/716 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 葛巻 暹
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