Size-based fungal growth studies have limitations. For example, the growth in size stops in closed systems once it reaches the borders and poorly describes metabolic status, especially in the stationary phase. This might lead mycotoxin studies to unrealistic results. Color change could be a viable alternative, as pigments result from a mold's metabolic activity. This study aimed to verify the possibility of using gray values and the RGB system to analyze the growth of Fusarium graminearum. It consisted of color and area measurements using ImageJ software for specimens grown in yeast extract agar (YEA). The results suggest the utility of color and gray values as reliable tools to analyze the growth of F. graminearum.