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Effects of human amnion-derived mesenchymal stem cells and conditioned medium in rats with sclerosing cholangitis

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Title: Effects of human amnion-derived mesenchymal stem cells and conditioned medium in rats with sclerosing cholangitis
Authors: Sugiura, Ryo Browse this author
Ohnishi, Shunsuke Browse this author →KAKEN DB
Ohara, Masatsugu Browse this author
Ishikawa, Marin Browse this author
Miyamoto, Shuichi Browse this author
Onishi, Reizo Browse this author
Yamamoto, Koji Browse this author
Kawakubo, Kazumichi Browse this author →KAKEN DB
Kuwatani, Masaki Browse this author →KAKEN DB
Sakamoto, Naoya Browse this author →KAKEN DB
Keywords: Mesenchymal stem cells
sclerosing cholangitis
regenerative medicine
Issue Date: 30-Jul-2018
Publisher: e-Century Publishing Corporation
Journal Title: American journal of translational research
Volume: 10
Issue: 7
Start Page: 2102
End Page: 2114
PMID: 30093947
Abstract: Mesenchymal stem cells (MSCs) represent a valuable cell source in regenerative medicine, and large numbers of MSCs can be isolated from the amnion noninvasively. Sclerosing cholangitis is a chronic cholestatic disease and characterized by progressive biliary destruction leading to cirrhosis. Many factors are involved in the development of sclerosing cholangitis; however, effective medical therapy is not established. We investigated the effects of human amnion-derived MSCs (hAMSCs) and conditioned medium (CM) obtained from hAMSC cultures in rats with sclerosing cholangitis. Sclerosing cholangitis was induced via the intragastric administration of 100 mg/kg alpha-naphthylisothiocyanate (ANIT) twice weekly for 4 weeks. One million hAMSCs or 200 mu L of CM were intravenously administered on days 15 and 22. Rats were sacrificed on day 29 and evaluated via histological, immunohistochemical, and mRNA expression analyses. hAMSC transplantation and CM administration significantly improved the histological score. In addition, these two interventions significantly improved biliary hyperplasia, peribiliary fibrosis, and inflammation in Glisson's sheath. Accordingly, CK19, MMP-9, and TNF-alpha, and MCP-1 expression in the liver was also decreased by hAMSC and CM administration. In conclusion, hAMSC and CM administration ameliorated biliary hyperplasia, peribiliary fibrosis, and inflammation in a rat model of sclerosing cholangitis. hAMSCs and CM may represent new modalities for treating sclerosing cholangitis.
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Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大西 俊介

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