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Effects of human amnion-derived mesenchymal stem cells and conditioned medium in rats with sclerosing cholangitis

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タイトル: Effects of human amnion-derived mesenchymal stem cells and conditioned medium in rats with sclerosing cholangitis
著者: Sugiura, Ryo 著作を一覧する
Ohnishi, Shunsuke 著作を一覧する
Ohara, Masatsugu 著作を一覧する
Ishikawa, Marin 著作を一覧する
Miyamoto, Shuichi 著作を一覧する
Onishi, Reizo 著作を一覧する
Yamamoto, Koji 著作を一覧する
Kawakubo, Kazumichi 著作を一覧する
Kuwatani, Masaki 著作を一覧する
Sakamoto, Naoya 著作を一覧する
キーワード: Mesenchymal stem cells
sclerosing cholangitis
amnion
alpha-naphthylisothiocyanate
regenerative medicine
発行日: 2018年 7月30日
出版者: e-Century Publishing Corporation
誌名: American journal of translational research
巻: 10
号: 7
開始ページ: 2102
終了ページ: 2114
PMID: 30093947
抄録: Mesenchymal stem cells (MSCs) represent a valuable cell source in regenerative medicine, and large numbers of MSCs can be isolated from the amnion noninvasively. Sclerosing cholangitis is a chronic cholestatic disease and characterized by progressive biliary destruction leading to cirrhosis. Many factors are involved in the development of sclerosing cholangitis; however, effective medical therapy is not established. We investigated the effects of human amnion-derived MSCs (hAMSCs) and conditioned medium (CM) obtained from hAMSC cultures in rats with sclerosing cholangitis. Sclerosing cholangitis was induced via the intragastric administration of 100 mg/kg alpha-naphthylisothiocyanate (ANIT) twice weekly for 4 weeks. One million hAMSCs or 200 mu L of CM were intravenously administered on days 15 and 22. Rats were sacrificed on day 29 and evaluated via histological, immunohistochemical, and mRNA expression analyses. hAMSC transplantation and CM administration significantly improved the histological score. In addition, these two interventions significantly improved biliary hyperplasia, peribiliary fibrosis, and inflammation in Glisson's sheath. Accordingly, CK19, MMP-9, and TNF-alpha, and MCP-1 expression in the liver was also decreased by hAMSC and CM administration. In conclusion, hAMSC and CM administration ameliorated biliary hyperplasia, peribiliary fibrosis, and inflammation in a rat model of sclerosing cholangitis. hAMSCs and CM may represent new modalities for treating sclerosing cholangitis.
資料タイプ: article
URI: http://hdl.handle.net/2115/71629
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 大西 俊介

 

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