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An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71638

Title: An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein
Authors: Furukawa, Takayuki Browse this author →KAKEN DB
Hinou, Hiroshi Browse this author →KAKEN DB
Takeda, Seiji Browse this author →KAKEN DB
Chiba, Hitoshi Browse this author →KAKEN DB
Nishimura, Shin-Ichiro Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Keywords: analytical methods
glycoblotting
lipids
lipoproteins
oxidation
Issue Date: 5-Oct-2017
Publisher: Wiley-Blackwell
Journal Title: Chembiochem
Volume: 18
Issue: 19
Start Page: 1903
End Page: 1909
Publisher DOI: 10.1002/cbic.201700384
PMID: 28779513
Abstract: Although widely occurring lipid oxidation, which is triggered by reactive oxygen species (ROS), produces a variety of oxidized lipids, practical methods to efficiently analyze oxidized lipids remain elusive. Herein, it is shown that the glycoblotting platform can be used to analyze oxidized lipids. Analysis is based on the principle that lipid aldehydes, one of the oxidized lipid species, can be captured selectively, enriched, and detected. Moreover, 3-methyl-1-p-tolyltriazene (MTT) methylates phosphoric and carboxylic acids, and this MTT-mediated methylation is, in combination with conventional tandem mass spectrometry (MS/MS) analysis, an effective method for the structural analysis of oxidized lipids. By using three classes of standards, liposomes, and a lipoprotein, it is demonstrated that glycoblotting represents a powerful approach for focused lipidomics, even in complex macromolecules.
Rights: This is the peer reviewed version of the following article: Furukawa, T., Hinou, H., Takeda, S., Chiba, H., Nishimura, SI., Hui, SP., An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein. Chembiochem, 2017; 18(19):1903-1909., which has been published in final form at 10.1002/cbic.201700384. This article may be used for non-commercial purposes in accordance with Wiley-VCH Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/71638
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 古川 貴之

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