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Whole-genome assembly of Babesia ovata and comparative genomics between closely related pathogens

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タイトル: Whole-genome assembly of Babesia ovata and comparative genomics between closely related pathogens
著者: Yamagishi, Junya 著作を一覧する
Asada, Masahito 著作を一覧する
Hakimi, Hassan 著作を一覧する
Tanaka, Takeshi Q. 著作を一覧する
Sugimoto, Chihiro 著作を一覧する
Kawazu, Shin-ichiro 著作を一覧する
キーワード: B. ovata
Babesia
MinION
Comparative genomics
発行日: 2017年10月27日
出版者: BioMed Central
誌名: BMC genomics
巻: 18
開始ページ: 832
出版社 DOI: 10.1186/s12864-017-4230-4
抄録: Background: Babesia ovata, belonging to the phylum Apicomplexa, is an infectious parasite of bovids. It is not associated with the manifestation of severe symptoms, in contrast to other types of bovine babesiosis caused by B. bovis and B. bigemina; however, upon co-infection with Theileria orientalis, it occasionally induces exacerbated symptoms. Asymptomatic chronic infection in bovines is usually observed only for B. ovata. Comparative genomic analysis could potentially reveal factors involved in these distinguishing characteristics; however, the genomic and molecular basis of these phenotypes remains elusive, especially in B. ovata. From a technical perspective, the current development of a very long read sequencer, MinION, will facilitate the obtainment of highly integrated genome sequences. Therefore, we applied next-generation sequencing to acquire a high-quality genome of the parasite, which provides fundamental information for understanding apicomplexans. Results: The genome was assembled into 14,453,397 bp in size with 5031 protein-coding sequences (91 contigs and N50 = 2,090,503 bp). Gene family analysis revealed that ves1 alpha and beta, which belong to multigene families in B. bovis, were absent from B. ovata, the same as in B. bigemina. Instead, ves1a and ves1b, which were originally specified in B. bigemina, were present. The B. ovata and B. bigemina ves1a configure one cluster together even though they divided into two sub-clusters according to the spp. In contrast, the ves1b cluster was more dispersed and the overlap among B. ovata and B. bigemina was limited. The observed redundancy and rapid evolution in sequence might reflect the adaptive history of these parasites. Moreover, same candidate genes which potentially involved in the distinct phenotypes were specified by functional analysis. An anamorsin homolog is one of them. The human anamorsin is involved in hematopoiesis and the homolog was present in B. ovata but absent in B. bigemina which causes severe anemia. Conclusions: Taking these findings together, the differences demonstrated by comparative genomics potentially explain the evolutionary history of these parasites and the differences in their phenotypes. Besides, the draft genome provides fundamental information for further characterization and understanding of these parasites.
資料タイプ: article
URI: http://hdl.handle.net/2115/71650
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 山岸 潤也

 

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