HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
遺伝子病制御研究所  >
雑誌発表論文等  >

The dominant negative H-ras mutant, N116Y, suppresses growth of metastatic

NK00Tak.pdf563.25 kBPDF見る/開く

タイトル: The dominant negative H-ras mutant, N116Y, suppresses growth of metastatic
その他のタイトル: Suppression of metastatic cancer by N116Y
著者: Takeuchi, M. 著作を一覧する
Shichinohe, T. 著作を一覧する
Senmaru, N. 著作を一覧する
Miyamoto, M. 著作を一覧する
Fujita, H. 著作を一覧する
Takimoto, M. 著作を一覧する
Kondo, S. 著作を一覧する
Katoh, H. 著作を一覧する
Kuzumaki, N. 著作を一覧する
キーワード: ras suppressor
pancreatic cancer
liver metastasis
発行日: 2000年 3月
出版者: Nature Publishing Group
誌名: Gene Therapy
巻: 7
号: 6
開始ページ: 518
終了ページ: 526
抄録: In pancreatic cancer, the mutation of c-K-ras is a critical event of tumor growth and metastasis.1 We have previously demonstrated a dominant negative effect of N116Y on the growth of pancreatic cancer cells.2 To evaluate the potential of N116Y for suppressing the metastatic growth of pancreatic tumor cells, we made a replication-deficient recombinant N116Y adenovirus driven by the carcinoembryonic antigen (CEA) promoter (Ad CEA-N116Y). We demonstrated that the expression of N116Y, growth inhibition, and apoptotic death induction were all specific to pancreatic cancer cell lines (PCI-35 and PCI-43) that were promoter positive, whereas no growth retardation was observed in human embryonic pancreas-derived cell line 1C3D3 after Ad CEA-N116Y infection. We examined the effect of Ad CEA-N116Y on the metastatic growth of PCI-43 colonies in liver, which were generated by tumor injection into the spleen of nude mice. The results showed that Ad CEA-N116Y effectively reduced the number of metastatic colonies without any complication by injecting intrasplenically five days after tumor cell inoculation. Thus N116Y can selectively suppress the metastatic growth of pancreatic tumor cell by using the CEA promoter driven adenovirus vector indicating that N116Y gene therapy may be potentially useful for the treatment of pancreatic cancer patients with liver micrometastasis.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 葛巻 暹


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学