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Quantitative imaging of pO2 in orthotopic murine gliomas : hypoxia correlates with resistance to radiation

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Title: Quantitative imaging of pO2 in orthotopic murine gliomas : hypoxia correlates with resistance to radiation
Authors: Yasui, Hironobu Browse this author →KAKEN DB
Kawai, Tatsuya Browse this author
Matsumoto, Shingo Browse this author
Saito, Keita Browse this author
Devasahayam, Nallathamby Browse this author
Mitchell, James B. Browse this author
Camphausen, Kevin Browse this author
Inanami, Osamu Browse this author →KAKEN DB
Krishna, Murali C. Browse this author
Keywords: Electron paramagnetic resonance imaging
Issue Date: 2017
Publisher: Taylor & Francis
Journal Title: Free Radical Research
Volume: 51
Issue: 9-10
Start Page: 861
End Page: 871
Publisher DOI: 10.1080/10715762.2017.1388506
PMID: 29076398
Abstract: Hypoxia is considered one of the microenvironmental factors associated with the malignant nature of glioblastoma. Thus, evaluating intratumoural distribution of hypoxia would be useful for therapeutic planning as well as assessment of its effectiveness during the therapy. Electron paramagnetic resonance imaging (EPRI) is an imaging technique which can generate quantitative maps of oxygen in vivo using the exogenous paramagnetic compound, triarylmethyl and monitoring its line broadening caused by oxygen. In this study, the feasibility of EPRI for assessment of oxygen distribution in the glioblastoma using orthotopic U87 and U251 xenograft model is examined. Heterogeneous distribution of pO2 between 0 and 50 mmHg was observed throughout the tumours except for the normal brain tissue. U251 glioblastoma was more likely to exhibit hypoxia than U87 for comparable tumour size (median pO2; 29.7 and 18.2 mmHg, p = .028, in U87 and U251, respectively). The area with pO2 under 10 mmHg on the EPR oximetry (HF10) showed a good correlation with pimonidazole staining among tumours with evaluated size. In subcutaneous xenograft model, irradiation was relatively less effective for U251 compared with U87. In conclusion, EPRI is a feasible method to evaluate oxygen distribution in the brain tumour.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Free Radical Research in 2017, available online:
Type: article (author version)
Appears in Collections:アイソトープ総合センター (Central Institute of Isotope Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 安井 博宣

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