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Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals

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Title: Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals
Authors: Yaguchi, Kan Browse this author
Yamamoto, Takahiro Browse this author
Matsui, Ryo Browse this author
Tsukada, Yuki Browse this author
Shibanuma, Atsuko Browse this author
Kamimura, Keiko Browse this author
Koda, Toshiaki Browse this author
Uehara, Ryota Browse this author →KAKEN DB
Keywords: Development
Issue Date: Jul-2018
Publisher: Rockefeller University Press
Journal Title: Journal of cell biology
Volume: 217
Issue: 7
Start Page: 2463
End Page: 2483
Publisher DOI: 10.1083/jcb.201701151
Abstract: In animals, somatic cells are usually diploid and are unstable when haploid for unknown reasons. In this study, by comparing isogenic human cell lines with different ploidies, we found frequent centrosome loss specifically in the haploid state, which profoundly contributed to haploid instability through subsequent mitotic defects. We also found that the efficiency of centriole licensing and duplication changes proportionally to ploidy level, whereas that of DNA replication stays constant. This caused gradual loss or frequent overduplication of centrioles in haploid and tetraploid cells, respectively. Centriole licensing efficiency seemed to be modulated by astral microtubules, whose development scaled with ploidy level, and artificial enhancement of aster formation in haploid cells restored centriole licensing efficiency to diploid levels. The ploidy-centrosome link was observed in different mammalian cell types. We propose that incompatibility between the centrosome duplication and DNA replication cycles arising from different scaling properties of these bioprocesses upon ploidy changes underlies the instability of non-diploid somatic cells in mammals.
Rights: ©Uehara, Ryota et al., 2018. Originally published in JOURNAL OF CELL BIOLOGY. doi:10.1083/jcb.201701151
Type: article
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 上原 亮太

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