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Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals

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タイトル: Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals
著者: Yaguchi, Kan 著作を一覧する
Yamamoto, Takahiro 著作を一覧する
Matsui, Ryo 著作を一覧する
Tsukada, Yuki 著作を一覧する
Shibanuma, Atsuko 著作を一覧する
Kamimura, Keiko 著作を一覧する
Koda, Toshiaki 著作を一覧する
Uehara, Ryota 著作を一覧する
キーワード: Development
Cytoskeleton
Cilia
発行日: 2018年 7月
出版者: Rockefeller University Press
誌名: Journal of cell biology
巻: 217
号: 7
開始ページ: 2463
終了ページ: 2483
出版社 DOI: 10.1083/jcb.201701151
抄録: In animals, somatic cells are usually diploid and are unstable when haploid for unknown reasons. In this study, by comparing isogenic human cell lines with different ploidies, we found frequent centrosome loss specifically in the haploid state, which profoundly contributed to haploid instability through subsequent mitotic defects. We also found that the efficiency of centriole licensing and duplication changes proportionally to ploidy level, whereas that of DNA replication stays constant. This caused gradual loss or frequent overduplication of centrioles in haploid and tetraploid cells, respectively. Centriole licensing efficiency seemed to be modulated by astral microtubules, whose development scaled with ploidy level, and artificial enhancement of aster formation in haploid cells restored centriole licensing efficiency to diploid levels. The ploidy-centrosome link was observed in different mammalian cell types. We propose that incompatibility between the centrosome duplication and DNA replication cycles arising from different scaling properties of these bioprocesses upon ploidy changes underlies the instability of non-diploid somatic cells in mammals.
Rights: ©Uehara, Ryota et al., 2018. Originally published in JOURNAL OF CELL BIOLOGY. doi:10.1083/jcb.201701151
資料タイプ: article
URI: http://hdl.handle.net/2115/71788
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 上原 亮太

 

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