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Ultradian calcium rhythms in the paraventricular nucleus and subparaventricular zone in the hypothalamus

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71975

Title: Ultradian calcium rhythms in the paraventricular nucleus and subparaventricular zone in the hypothalamus
Authors: Wu, Yu-Er Browse this author
Enoki, Ryosuke Browse this author →KAKEN DB
Oda, Yoshiaki Browse this author
Huang, Zhi-Li Browse this author
Honma, Ken-ichi Browse this author
Honma, Sato Browse this author
Keywords: intracellular calcium
fluorescence imaging
neuronal network
ultradian rhythm
circadian rhythm
Issue Date: 2-Oct-2018
Publisher: National Academy of Sciences.
Journal Title: Proceedings of the National Academy of Sciences of the United States of America (PNAS)
Volume: 115
Issue: 40
Start Page: E9469
End Page: E9478
Publisher DOI: 10.1073/pnas.1804300115
Abstract: The suprachiasmatic nucleus (SCN), the master circadian clock in mammals, sends major output signals to the subparaventricular zone (SPZ) and further to the paraventricular nucleus (PVN), the neural mechanism of which is largely unknown. In this study, the intracellular calcium levels were measured continuously in cultured hypothalamic slices containing the PVN, SPZ, and SCN. We detected ultradian calcium rhythms in both the SPZ-PVN and SCN regions with periods of 0.5-4.0 hours, the frequency of which depended on the local circadian rhythm in the SPZ-PVN region. The ultradian rhythms were synchronous in the entire SPZ-PVN region and a part of the SCN. Because the ultradian rhythms were not detected in the SCN-only slice, the origin of ultradian rhythm is the SPZ-PVN region. In association with an ultradian bout, a rapid increase of intracellular calcium in a millisecond order was detected, the frequency of which determined the amplitude of an ultradian bout. The synchronous ultradian rhythms were desynchronized and depressed by a sodium channel blocker tetrodotoxin, suggesting that a tetrodotoxin-sensitive network is involved in synchrony of the ultradian bouts. In contrast, the ultradian rhythm is abolished by glutamate receptor blockers, indicating the critical role of glutamatergic mechanism in ultradian rhythm generation, while a GABA(A) receptor blocker increased the frequency of ultradian rhythm and modified the circadian rhythm in the SCN. A GABAergic network may refine the circadian output signals. The present study provides a clue to unraveling the loci and network mechanisms of the ultradian rhythm.
Rights: https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article
URI: http://hdl.handle.net/2115/71975
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 榎木 亮介

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