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Ultradian calcium rhythms in the paraventricular nucleus and subparaventricular zone in the hypothalamus
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| Title: | Ultradian calcium rhythms in the paraventricular nucleus and subparaventricular zone in the hypothalamus |
| Authors: | Wu, Yu-Er Browse this author | | Enoki, Ryosuke Browse this author →KAKEN DB | | Oda, Yoshiaki Browse this author | | Huang, Zhi-Li Browse this author | | Honma, Ken-ichi Browse this author | | Honma, Sato Browse this author |
| Keywords: | intracellular calcium | | fluorescence imaging | | neuronal network | | ultradian rhythm | | circadian rhythm |
| Issue Date: | 2-Oct-2018 |
| Publisher: | National Academy of Sciences. |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America (PNAS) |
| Volume: | 115 |
| Issue: | 40 |
| Start Page: | E9469 |
| End Page: | E9478 |
| Publisher DOI: | 10.1073/pnas.1804300115 |
| Abstract: | The suprachiasmatic nucleus (SCN), the master circadian clock in mammals, sends major output signals to the subparaventricular zone (SPZ) and further to the paraventricular nucleus (PVN), the neural mechanism of which is largely unknown. In this study, the intracellular calcium levels were measured continuously in cultured hypothalamic slices containing the PVN, SPZ, and SCN. We detected ultradian calcium rhythms in both the SPZ-PVN and SCN regions with periods of 0.5-4.0 hours, the frequency of which depended on the local circadian rhythm in the SPZ-PVN region. The ultradian rhythms were synchronous in the entire SPZ-PVN region and a part of the SCN. Because the ultradian rhythms were not detected in the SCN-only slice, the origin of ultradian rhythm is the SPZ-PVN region. In association with an ultradian bout, a rapid increase of intracellular calcium in a millisecond order was detected, the frequency of which determined the amplitude of an ultradian bout. The synchronous ultradian rhythms were desynchronized and depressed by a sodium channel blocker tetrodotoxin, suggesting that a tetrodotoxin-sensitive network is involved in synchrony of the ultradian bouts. In contrast, the ultradian rhythm is abolished by glutamate receptor blockers, indicating the critical role of glutamatergic mechanism in ultradian rhythm generation, while a GABA(A) receptor blocker increased the frequency of ultradian rhythm and modified the circadian rhythm in the SCN. A GABAergic network may refine the circadian output signals. The present study provides a clue to unraveling the loci and network mechanisms of the ultradian rhythm. |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71975 |
| Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 榎木 亮介
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