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Cloning of the Bisucaberin B Biosynthetic Gene Cluster from the Marine Bacterium Tenacibaculum mesophilum, and Heterologous Production of Bisucaberin B

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Mar. Drugs 2018, 16(9), 342.pdf1.72 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71976

Title: Cloning of the Bisucaberin B Biosynthetic Gene Cluster from the Marine Bacterium Tenacibaculum mesophilum, and Heterologous Production of Bisucaberin B
Authors: Fujita, Masaki J. Browse this author →KAKEN DB
Goto, Yusuke Browse this author
Sakai, Ryuichi Browse this author
Keywords: siderophore
biosynthesis
heterologous production
bisucaberin
desferrioxamine
Issue Date: 19-Sep-2018
Publisher: MDPI
Journal Title: Marine drugs
Volume: 16
Issue: 9
Start Page: 342
Publisher DOI: 10.3390/md16090342
Abstract: The biosynthetic gene cluster for bisucaberin B (1, bsb gene cluster), an N-hydroxy-N-succinyl diamine (HSD)-based siderophore, was cloned from the marine bacterium Tenacibaculum mesophilum, originated from a marine sponge. The bsb gene cluster consists of six open reading frames (ORFs), in contrast to the four ORFs typically seen in biosynthetic gene clusters of the related molecules. Heterologous expression of the key enzyme, BsbD2, which is responsible for the final biosynthetic step of 1 resulted in production of bisucaberin B (1), but not bisucaberin (2) a macrocyclic counterpart of 1. To date, numbers of related enzymes producing macrocyclic analogues have been reported, but this work represents the first example of the HSD-based siderophore biosynthetic enzyme which exclusively produces a linear molecule rather than macrocyclic counterparts.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/71976
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 藤田 雅紀

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