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Inhibition of apoptosis by the actin-regulatory protein gelsolin

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Title: Inhibition of apoptosis by the actin-regulatory protein gelsolin
Authors: Ohtsu, Makoto Browse this author
Sakai, Norio Browse this author
Fujita, Hisakazu Browse this author
Kashiwagi, Motoi Browse this author
Gasa, Shinsei Browse this author
Shimizu, Shigeomi Browse this author
Eguchi, Yutaka Browse this author
Tsujimoto, Yoshihide Browse this author
Sakiyama, Yukio Browse this author
Kobayashi, Kunihiko Browse this author
Kuzumaki, Noboru11 Browse this author
Authors(alt): 葛巻, 暹11
Keywords: apoptosis
CPP32
Fas
gelsolin
Jurkat cells
Issue Date: Aug-1997
Publisher: Nature Publishing Group
Journal Title: The EMBO Journal
Volume: 16
Issue: 15
Start Page: 4650
End Page: 4656
Abstract: Gelsolin is an actin-regulatory protein that modulates actin assembly and disassembly, and is believed to regulate cell motility invivo through modulation of the actin network. In addition to its actin-regulatory function, gelsolin has also been proposed to affect cell growth. Our present experiments have tested the possible involvement of gelsolin in the regulation of apoptosis, which is significantly affected by growth. When overexpressed in Jurkat cells, gelsolin strongly inhibited apoptosis induced by anti-Fas antibody, C2-ceramide or dexamethasone, without changing the F-actin morphology or the levels of Fas or Bcl-2 family proteins. Upon the induction of apoptosis, an increase in CPP32(-like) protease activity was observed in the control vector transfectants, while it was strongly suppressed in the gelsolin transfectants. Pro-CPP32 protein, an inactive form of CPP32 protease, remained uncleaved by anti-Fas treatment in the gelsolin transfectants, indicating that gelsolin blocks upstream of this protease. The tetrapeptide inhibitor of CPP32(-like) proteases strongly inhibited Fas-mediated apoptosis, but only partially suppressed both C2-ceramide- and dexamethasone-induced apoptosis. These data suggest that the critical target responsible for the execution of apoptosis may exist upstream of CPP32(-like) proteases in Jurkat cells and that gelsolin acts on this target to inhibit the apoptotic cell death program.
Type: article (author version)
URI: http://embojournal.npgjournals.com/
http://hdl.handle.net/2115/720
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 葛巻 暹

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